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Evaluation of Paste-Type Micronized Acellular Dermal Matrix for Soft Tissue Augmentation: Volumetric and Histological Assessment in a Mouse Model

  • Original Article
  • Basic Science/Experimental
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Abstract

Background

A biological injectable material, paste-type micronized acellular dermal matrix (ADM), has been proven effective in wound healing by filling defects through tissue replacement. This study aimed to compare the efficacy of paste-type micronized ADM on soft tissue augmentation with that of the conventional fillers in animal experiments.

Methods

Two distinct paste-type micronized ADMs, which were mixed with distilled water (mADM) and gelatin (mADM+GEL), respectively, were compared with conventional fillers, hyaluronic acid (HA) and polymethyl methacrylate (COL+PMMA). Thus, four different types of fillers were each injected into the dorsum of nude mice to compare the volume retention and biocompatibility. During the 8-week experimental period, ultrasound and computed tomography (CT) images were obtained for volumetric analysis. Histological evaluation was performed using hematoxylin and eosin and CD 31 staining.

Results

According to the CT images at week 8, the mADM and mADM+GEL showed a higher volume persistence rate of 113.54% and 51.12%, compared with 85.09% and 17.65% for HA and COL+PMMA, respectively. The 2-week interval ultrasound images revealed that the mADM showed a volume increase in width rather than in height, and an increase in height for HA did not vary much. Histological analysis showed marked fibrous invasion and neovascularization with the mADM and mADM+GEL compared to that of the conventional fillers.

Conclusions

Paste-type micronized ADM showed soft tissue augmentation with similar effectiveness to that of conventional fillers. Therefore, paste-type micronized ADM has potential as an alternative material for a soft tissue filler in tissue replacement.

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References

  1. Sclafani AP, Romo T III (2000) Injectable fillers for facial soft tissue enhancement. Facial Plast Surg 16(01):29–34

    Article  CAS  PubMed  Google Scholar 

  2. Sclafani AP, Romo T III (2001) Collagen, human collagen, and fat: the search for a three-dimensional soft tissue filler. Facial Plast Surg 17(01):079–085

    Article  CAS  Google Scholar 

  3. Achauer BM, VanderKam VM, Celikoz B, Jacobson DG (1998) Augmentation of facial soft-tissue defects with Alloderm dermal graft. Ann Plast Surg 41(5):503–507

    Article  CAS  PubMed  Google Scholar 

  4. Ibrahim AM, Ayeni OA, Hughes KB, Lee BT, Slavin SA, Lin SJ (2013) Acellular dermal matrices in breast surgery: a comprehensive review. Ann Plast Surg 70(6):732–738

    Article  CAS  PubMed  Google Scholar 

  5. Sherris DA, Oriel BS (2011) Human acellular dermal matrix grafts for rhinoplasty. Aesthet Surg J 31(7_Supplement):95S-100S

    Article  PubMed  Google Scholar 

  6. Banta MN, Eaglstein WH, Kirsner RS (2003) Healing of refractory sinus tracts by dermal matrix injection with Cymetra. Dermatol Surg 29(8):863–866

    PubMed  Google Scholar 

  7. Kim YH, Shim HS, Lee J, Kim SW (2022) A prospective randomized controlled multicenter clinical trial comparing paste-type acellular dermal matrix to standard care for the treatment of chronic wounds. J Clin Med 11(8):2203

    Article  PubMed  PubMed Central  Google Scholar 

  8. Jeon M, Kim SY (2018) Application of a paste-type acellular dermal matrix for coverage of chronic ulcerative wounds. Arch Plast Surg 45(06):564–571

    Article  PubMed  PubMed Central  Google Scholar 

  9. Jang HJ, Kim YM, Yoo BY, Seo YK (2018) Wound-healing effects of human dermal components with gelatin dressing. J Biomater Appl 32(6):716–724

    Article  CAS  PubMed  Google Scholar 

  10. Kim JH, Kim SE, Kim YJ, Kim YW, Cheon YW (2020) Comparison of volume retention and biocompatibility of acellular dermal matrix/hyaluronic acid filler to autologous fat grafts in a mouse model. Aesthet Plast Surg 44(3):986–992

    Article  Google Scholar 

  11. Breiman RS, Beck JW, Korobkin M, Glenny R, Akwari OE, Heaston DK, Ram PC (1982) Volume determinations using computed tomography. AJR Am J Roentgenol 138(2):329–333

    Article  CAS  PubMed  Google Scholar 

  12. Lee YB, Park SM, Song EJ, Park JG, Cho KO, Kim JW, Yu DS (2014) Histology of a novel injectable filler (polymethylmethacrylate and cross-linked dextran in hydroxypropyl methylcellulose) in a rat model. J Cosmet Laser Ther 16(4):191–196

    Article  CAS  PubMed  Google Scholar 

  13. Iorio ML, Shuck J, Attinger CE (2012) Wound healing in the upper and lower extremities: a systematic review on the use of acellular dermal matrices. Plast Reconstr Surg 130(5S–2):232S-241S

    Article  CAS  PubMed  Google Scholar 

  14. Tognetti L, Pianigiani E, Ierardi F, Lorenzini G, Casella D, Liso FG, Rubegni P (2021) The use of human acellular dermal matrices in advanced wound healing and surgical procedures: State of the art. Dermatol Ther 34(4):e14987

    Article  PubMed  Google Scholar 

  15. Pietramaggiori G, Scherer SS, Mathews JC, Alperovich M, Yang HJ, Neuwalder J, Orgill DP (2008) Healing modulation induced by freeze-dried platelet-rich plasma and micronized allogenic dermis in a diabetic wound model. Wound Repair Regen 16(2):218–225

    Article  PubMed  Google Scholar 

  16. Hee CK, Shumate GT, Narurkar V, Bernardin A, Messina DJ (2015) Rheological properties and in vivo performance characteristics of soft tissue fillers. Dermatol Surg 41:S373–S381

    Article  PubMed  Google Scholar 

  17. Sclafani AP, Romo T III, Jacono AA, McCormick S, Cocker R, Parker A (2000) Evaluation of acellular dermal graft in sheet (AlloDerm) and injectable (micronized AlloDerm) forms for soft tissue augmentation: clinical observations and histological analysis. Arch Facial Plast Surg 2(2):130–136

    Article  CAS  PubMed  Google Scholar 

  18. Lee HY, Yang HJ, Rhie JW, Han KT (2014) Adipose tissue regeneration in vivo using micronized acellular allogenic dermis as an injectable scaffold. Aesthet Plast Surg 38(5):1001–1010

    Article  Google Scholar 

  19. Wise JB, Cabiling D, Yan D, Mirza N, Kirschner RE (2007) Submucosal injection of micronized acellular dermal matrix: analysis of biocompatibility and durability. Plast Reconstr Surg 120(5):1156–1160

    Article  CAS  PubMed  Google Scholar 

  20. Choi YS, Hong SR, Lee YM, Song KW, Park MH, Nam YS (1999) Study on gelatin-containing artificial skin: I. Preparation and characteristics of novel gelatin-alginate sponge. Biomaterials 20(5):409–417

    Article  CAS  PubMed  Google Scholar 

  21. Tuin A, Kluijtmans SG, Bouwstra JB, Harmsen MC, Van Luyn MJ (2010) Recombinant gelatin microspheres: novel formulations for tissue repair? Tissue Eng Part A 16(6):1811–1821

    Article  CAS  PubMed  Google Scholar 

  22. Olsen D, Yang C, Bodo M, Chang R, Leigh S, Baez J, Polarek J (2003) Recombinant collagen and gelatin for drug delivery. Adv Drug Deliv Rev 55(12):1547–1567

    Article  CAS  PubMed  Google Scholar 

  23. Cohen SR, Holmes RE (2004) Artecoll: a long-lasting injectable wrinkle filler material: report of a controlled, randomized, multicenter clinical trial of 251 subjects. Plast Reconstr Surg 114(4):964–976

    Article  PubMed  Google Scholar 

  24. Chen L, Li SR, Yu P, Wang ZX (2014) Comparison of Artecoll, Restylane and silicone for augmentation rhinoplasty in 378 Chinese patients. Clin Invest Med, E203-E210.

  25. Lemperle G, Ott H, Charrier U, Hecker J, Lemperle M (1991) PMMA microspheres for intradermal implantation—part I. Animal research. Ann Plast Surg 26(1):57–63

    Article  CAS  PubMed  Google Scholar 

  26. Gold MH, Sadick NS (2018) Optimizing outcomes with polymethylmethacrylate fillers. J Cosmet Dermatol 17(3):298–304

    Article  PubMed  Google Scholar 

  27. Pollack SV (1999) Some new injectable dermal filler materials: Hylaform, Restylane, and Artecoll. J Cutan Med Surg 3(4_suppl):S4-27

    Article  Google Scholar 

  28. Choi JS, Yang HJ, Kim BS, Kim JD, Kim JY, Yoo B, Cho YW (2009) Human extracellular matrix (ECM) powders for injectable cell delivery and adipose tissue engineering. J Control Release 139(1):2–7

    Article  CAS  PubMed  Google Scholar 

  29. Jeong SH, Fan YF, Baek JU, Song J, Choi TH, Kim SW, Kim HE (2016) Long-lasting and bioactive hyaluronic acid-hydroxyapatite composite hydrogels for injectable dermal fillers: physical properties and in vivo durability. J Biomater Appl 31(3):464–474

    Article  CAS  PubMed  Google Scholar 

  30. Yoo G, Lim JS (2009) Tissue engineering of injectable soft tissue filler: using adipose stem cells and micronized acellular dermal matrix. J Korean Med Sci 24(1):104–109

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgement

This research was supported by Young Medical Scientist Research Grant through the Daewoong Foundation (DY18205P)

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Author notes

  1. Young Bin Choy and Sang Wha Kim have contributed equal to this work

Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, College of Medicine, Hanyang University, Seoul, Republic of Korea

    Lan Sook Chang

  2. Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea

    Seung Hwan Kim & Sang Wha Kim

  3. Department of Pathology, Hanyang University College of Medicine, Seoul, Republic of Korea

    Hyunsung Kim

  4. Biostatistical Consulting and Research Lab, Medical Research Collaborating Center, Hanyang University, Seoul, Republic of Korea

    Soorack Ryu

  5. Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea

    Young Bin Choy

  6. Medical Research Center, Institute of Medical and Biological Engineering, Seoul National University, Seoul, Republic of Korea

    Young Bin Choy

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  1. Lan Sook Chang
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Correspondence to Young Bin Choy or Sang Wha Kim.

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Chang, L.S., Kim, S.H., Kim, H. et al. Evaluation of Paste-Type Micronized Acellular Dermal Matrix for Soft Tissue Augmentation: Volumetric and Histological Assessment in a Mouse Model. Aesth Plast Surg 47, 852–861 (2023). https://doi.org/10.1007/s00266-022-03051-x

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  • DOI: https://doi.org/10.1007/s00266-022-03051-x

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