Abstract
Quantification of animal colors is important to a variety of fields of research, especially those dealing with visual communication and sexual selection. Most animal colors are easily measured using well-established spectrophotometric techniques. However, the unique characteristics of iridescent colors present particular challenges and opportunities to quantify novel color metrics. Due to the fine-scale angle dependence of iridescent coloration, color metrics, such as hue and brightness, must be measured using methods that allow for repeatable comparison across individuals (e.g., by carefully controlling and measuring viewing geometry). Here, we explain how the optical characteristics of iridescent colors should be considered when developing measurement techniques, describe the pitfalls of some commonly used techniques, and recommend improved methods and metrics (angular degree of color change and breadth of reflectance) for quantifying iridescent color. In particular, most studies of iridescent birds to date have used less than ideal procedures and have not provided repeatability estimates for their methods. For example, we demonstrate here that measuring coloration from overlapping patches of iridescent feathers may be problematic, and we argue against methods that do not carefully control viewing geometry. We recommend measuring iridescence at maximal reflectance angles using an apparatus that allows for sample rotation, and we compare this technique to some other commonly used methods using iridescent gorget and crown feathers from Anna’s hummingbirds (Calypte anna). Our apparatus allows for the quantification of angular color change, and we found that maximal reflectance measurements using single feathers are highly repeatable both within feather samples and among samples within an individual.
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References
Andersson S, Prager M (2006) Quantifying colors. In: Hill GE, McGraw KJ (eds) Bird coloration: mechanisms and measurements. Harvard University Press, Cambridge, pp 41–89
Baribeau R, Neil WS, Cote E (2009) Development of a robot-based gonioreflectometer for spectral BRDF measurement. J Mod Opt 56:1497–1503
Bennett ATD, Cuthill IC, Norris KJ (1994) Sexual selection and the mismeasure of color. Am Nat 144:848–860
Biro LP, Vigneron JP (2011) Photonic nanoarchitectures in butterflies and beetles: valuable sources for bioinspiration. Laser Photon Rev 5:27–51
Bitton P, Dawson RD (2008) Age-related differences in plumage characteristics of male tree swallows Tachycineta bicolor: hue and brightness signal different aspects of individual quality. J Avian Biol 39:446–452
Bitton P, O’Brien EL, Dawson RD (2007) Plumage brightness and age predict extrapair fertilization success of male tree swallows, Tachycineta bicolor. Anim Behav 74:1777–1784
Bitton P, Dawson RD, Ochs CL (2008) Plumage characteristics, reproductive investment and assortative mating in tree swallows Tachycineta bicolor. Behav Ecol Sociobiol 62:1543–1550
Cornwall C, Horiuchi A, Lehman C (2009) National oceanic and atmospheric administration solar position calculator. http://www.srrb.noaa.gov/highlights/sunrise/axel.html. Accessed Jul 2009
Costa FJV, Macedo RH (2005) Coccidian oocyst parasitism in the blue-black grassquit: influence on secondary sex ornaments and body condition. Anim Behav 70:1401–1409
Cuthill IC (2006) Color perception. In: Hill GE, McGraw KJ (eds) Bird coloration: mechanisms and measurements. Harvard University Press, Cambridge, pp 3–40
Cuthill IC, Bennett ATD, Partridge JC, Maier EJ (1999) Plumage reflectance and the objective assessment of avian sexual dichromatism. Am Nat 153:183–200
Doucet SM (2002) Structural plumage coloration, male body size, and condition in the blue-black grassquit. Condor 104:30–38
Doucet SM, Meadows MG (2009) Iridescence: a functional perspective. J R Soc Interface 6:S115–S132
Doucet SM, Shawkey MD, Hill GE, Montgomerie R (2006) Iridescent plumage in satin bowerbirds: structure, mechanisms and nanostructural predictors of individual variation in colour. J Exp Biol 209:380–390
Greenewalt CH, Brandt W, Friel DD (1960) Iridescent colors of hummingbird feathers. J Opt Soc Am 50:1005–1013
Hamilton WJ (1965) Sun-oriented display of the Anna’s hummingbird. Wilson Bull 77:38–44
Hill GE, McGraw KJ (2006a) Bird coloration: mechanisms and measurements. Harvard University Press, Cambridge
Hill GE, McGraw KJ (2006b) Bird coloration: function and evolution. Harvard University Press, Cambridge
Hill GE, Doucet SM, Buchholz R (2005) The effect of coccidial infection on iridescent plumage coloration in wild turkeys. Anim Behav 69:387–394
Huxley AF (1968) A theoretical treatment of reflexion of light by multilayer structures. J Exp Biol 48:227–245
Kemp DJ (2006a) Heightened phenotypic variation and age-based fading of ultraviolet butterfly wing coloration. Evol Ecol Res 8:515–527
Kemp DJ (2006b) Ultraviolet ornamentation and male mating success in a high-density assemblage of the butterfly Colias eurytheme. J Insect Behav 19:669–684
Kemp DJ (2008a) Female mating biases for bright ultraviolet iridescence in the butterfly Eurema hecabe (Pieridae). Behav Ecol 19:1–8
Kemp DJ (2008b) Resource-mediated condition dependence in sexually dichromatic butterfly wing coloration. Evolution 62:2346–2358
Kemp DJ, Macedonia JM (2006) Structural ultraviolet ornamentation in the butterfly Hypolimnas bolina L. (Nymphalidae): visual, morphological and ecological properties. Aust J Zool 54:235–244
Kemp DJ, Rutowski RL (2007) Condition dependence, quantitative genetics, and the potential signal content of iridescent ultraviolet butterfly coloration. Evolution 61:168–183
Kemp DJ, Vukusic P, Rutowski RL (2006) Stress-mediated covariance between nano-structural architecture and ultraviolet butterfly coloration. Funct Ecol 20:282–289
Kemp DJ, Macedonia JM, Ball TS, Rutowski RL (2008) Potential direct fitness consequences of ornament-based mate choice in a butterfly. Behav Ecol Sociobiol 62:1017–1026
Kinoshita S, Yoshioka S, Miyazaki J (2008) Physics of structural colors. Rep Prog Phys 71:1–30
Land MF (1972) The physics and biology of animal reflectors. Prog Biophys Mol Biol 24:77–106
Lessells CM, Boag PT (1987) Unrepeatable repeatabilities: a common mistake. Auk 104:116–121
Lim MLM, Li D (2006) Extreme ultraviolet sexual dimorphism in jumping spiders (Araneae: Salticidae). Biol J Linn Soc 89:397–406
Loyau A, Gomez D, Moureau B, Thery M, Hart NS, Saint Jalme M, Bennett ATD, Sorci G (2007) Iridescent structurally based coloration of eyespots correlates with mating success in the peacock. Behav Ecol 18:1123–1131
Madsen V, Dabelsteen T, Osorio D, Osorno JL (2007) Morphology and ornamentation in male magnificent frigatebirds: variation with age class and mating status. Am Nat 169:S93–S111
Meadows MG, Butler MW, Morehouse NI, Taylor LA, Toomey MB, McGraw KJ, Rutowski RL (2009) Iridescence: views from many angles. J R Soc Interface 6:S107–S113
Montgomerie R (2006) Analyzing colors. In: Hill GE, McGraw KJ (eds) Bird coloration: mechanisms and measurements. Harvard University Press, Cambridge, pp 90–147
Montgomerie R (2008) CLR, version 1.05. Queen’s University, Kingston, Canada. http://post.queensu.ca/∼mont/color/analyze.html. Accessed 2 Dec 2008
Mullen P, Pohland G (2008) Studies on UV reflection in feathers of some 1000 bird species: are UV peaks in feathers correlated with violet-sensitive and ultraviolet-sensitive cones? Ibis 150:59–68
Osorio D, Ham AD (2002) Spectral reflectance and directional properties of structural coloration in bird plumage. J Exp Biol 205:2017–2027
Papke RS, Kemp DJ, Rutowski RL (2007) Multimodal signalling: structural ultraviolet reflectance predicts male mating success better than pheromones in the butterfly Colias eurytheme L. (Pieridae). Anim Behav 73:47–54
Prum RO (2006) Anatomy, physics, and evolution of structural colors. In: Hill GE, McGraw KJ (eds) Bird coloration: mechanisms and measurements. Harvard University Press, Cambridge, pp 295–355
Rutowski RL, Macedonia JM, Morehouse N, Taylor-Taft L (2005) Pterin pigments amplify iridescent ultraviolet signal in males of the orange sulphur butterfly, Colias eurytheme. Proc R Soc B Lond 272:2329–2335
Rutowski RL, Macedonia JM, Merry JW, Morehouse NI, Yturralde K, Taylor-Taft L, Gaalema D, Kemp DJ, Papke RS (2007) Iridescent ultraviolet signal in the orange sulphur butterfly (Colias eurytheme): spatial, temporal and spectral properties. Biol J Linn Soc 90:349–364
Rutowski RL, Nahm A, Macedonia JM (2010) Iridescent hindwing patches in the pipevine swallowtail: differences in dorsal and ventral surfaces relate to signal function and context. Funct Ecol 24:767–775
Safran RJ, McGraw KJ (2004) Plumage coloration, not length or symmetry of tail-streamers, is a sexually selected trait in North American barn swallows. Behav Ecol 15:455–461
Santos ESA, Maia R, Macedo RH (2009) Condition-dependent resource value affects male–male competition in the blue-black grassquit. Behav Ecol 20:553–559
Shawkey MD, Hill GE (2005) Carotenoids need structural colours to shine. Biol Lett 1:121–124
Stiles FG (1982) Aggressive and courtship displays of the male Anna’s hummingbird. Condor 84:208–225
Vukusic P, Stavenga DG (2009) Physical methods for investigating structural colours in biological systems. J R Soc Interface 6:S133–S148
Vukusic P, Sambles JR, Lawrence CR, Wootton RJ (2002) Limited-view iridescence in the butterfly Ancyluris meliboeus. Proc R Soc Lond B 269:7–14
Vukusic P, Wootton RJ, Sambles JR (2004) Remarkable iridescence in the hindwings of the damselfly Neurobasis chinensis chinensis (Linnaeus) (Zygoptera: Calopterygidae). Proc R Soc Lond B 271:595–601
Wolfenbarger LL (1999) Is red coloration of male Northern Cardinals beneficial during the nonbreeding season?: a test of status signaling. Condor 101:655–663
Zar JH (1999) Biostatistical analysis. Prentice-Hall, Upper Saddle River
Acknowledgments
We thank Richard Flubacher and the Arizona State University Engineering Machine Shop for patiently helping us design and build our apparatus and Joe Macedonia for contributions to the design of the apparatus. We thank Daniel Osorio for sharing his advice and diagrams of his color measurement apparatus. Three anonymous reviewers greatly improved the quality of this manuscript. MGM was supported by a National Science Foundation Graduate Research Fellowship during the completion of this manuscript. Funding was provided by the College of Liberal Arts and Sciences and School of Life Sciences at Arizona State University.
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Communicated by L. Garamszegi
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Meadows, M.G., Morehouse, N.I., Rutowski, R.L. et al. Quantifying iridescent coloration in animals: a method for improving repeatability. Behav Ecol Sociobiol 65, 1317–1327 (2011). https://doi.org/10.1007/s00265-010-1135-5
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DOI: https://doi.org/10.1007/s00265-010-1135-5