Abstract
Purpose
The purpose of this study was to investigate the possible association between single nucleotide polymorphisms (SNPs) rs1800629 (TNF-α -308) and rs361525 (TNF-α -238) of the tumour necrosis factor (TNF)-α gene and susceptibility to osteoarthritis (OA) in the Han Chinese population.
Methods
The TNF-α -308 and -238 genotypes were determined by TaqMan assay in 200 OA cases and 305 controls. Odds ratios (ORs) for OA and 95 % confidence intervals (CIs) from unconditional logistic regression models were used to evaluate relative risks.
Results
The frequencies of the allele ‘A’ of rs1800629 were 16 % and 8.85 % in OA cases and in controls, respectively, and thus the -308A allele had a 1.9612-fold (95 % CI = 1.3323–2.8869, P < 0.001) increased risk for OA as compared to the -308G allele. However, no significant differences were found in the genotype and allele frequencies for rs361525 between OA and HC groups.
Conclusions
In the Han Chinese population, the allele ‘A’ of TNF-α -308 may increase the risk for OA, whereas TNF-α -238 polymorphisms do not play a role in OA patients.
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Acknowledgments
This study was supported by grants from the Shanghai Municipal Health Bureau Program, China (No. 2010164), National Key Constructive Disciplines of China (No.T0303), and the Cultivation Program of “Advanced Reserve Expert”, General Practice of Traditional Chinese Medicine, the Key Disciplines of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine in "Twelfth Five-Year" Plan, Shanghai University of Traditional Chinese Medicine, China. The authors wish to thank Dr. Zhongzheng Zhu, Dr. Jianpeng Li, Dr. Yuxiang Jiang and Dr. Yan Zhou for excellent technical assistance.
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Bin Ji and Jixiang Shi contributed equally to the work.
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Ji, B., Shi, J., Cheng, X. et al. Association analysis of two candidate polymorphisms in the Tumour Necrosis Factor-α gene with osteoarthritis in a Chinese population. International Orthopaedics (SICOT) 37, 2061–2063 (2013). https://doi.org/10.1007/s00264-013-1931-4
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DOI: https://doi.org/10.1007/s00264-013-1931-4