Abstract
This study investigated the ability of optical coherence tomography (OCT), a recently developed technology with micron-scale resolution, to assess the microstructure of tendons and ligaments. In vitro structural- and polarization-sensitive OCT was performed on human ACL, Achilles tendon, and biceps tendon (obtained postmortem). Histology was performed on all imaged samples and compared to the corresponding OCT data. OCT images correlated well with histology. Most importantly, through polarization-sensitive OCT, the collagen in normal tissue was easily distinguished from the surrounding, supportive tissue due to the birefringent properties of organized collagen. Since the integrity of collagen is an important indicator of structural stability and pathologic state, the ability of OCT to assess collagen could be a powerful diagnostic tool in assessing tendon and ligament properties.
Résumé
Cette étude a examiné la capacité de la tomographie de cohésion optique (OCT), une technologie récemment développée avec une résolution de l'échelle du micron, à étudier la microstructure des tendons et ligaments. L'étude in vitro a été exécuté sur le ligament croisé antérieur humain, le tendon d'Achille et le tendon du biceps ( obtenu post mortem). L'histologie a été exécutée sur tous les échantillons et comparée aux données OCT correspondantes. Les images OCT ont eu une bonne corrélation avec l'histologie. Plus important, à travers l'image OCT en polarisation, le collagène du tissu normal a été distingué facilement des tissus avoisinants grace à ses propriétés biréfringentes. Puisque l'intégrité du collagène est un indicateur important de la stabilité structurelle ou d'un état pathologique, la capacité de l'OCT à apprécier le collagène pourrait être un outil diagnostique puissant dans l'étude des propriétés des tendons et ligaments.
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Acknowledgments
This research is supported in part by the National Institutes of Health, Contract NIH R01 HL63953–01 (MEB), NIH R01 AR46996–01 (MEB), NIH-9-RO1-EY11289–14 (JGF), NIH-1-RO1-CA75289–04 (JGF); the Medical Free Electron Laser Program, Office of Naval Research Contract Grant F49620–01–1-0186 (JGF and MEB); and the National Institutes of Health, Contracts 1RO1AR44812–01 (MEB), NIH-1-R29-HL55686–01A1 (MEB), NIH-RO1-AR44812–02 (MEB), R01 HL55686–07 (MEB), and R01EB000419–01A1 (MEB).
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Martin, S.D., Patel, N.A., Adams, S.B. et al. New technology for assessing microstructural components of tendons and ligaments. International Orthopaedics (SICOT) 27, 184–189 (2003). https://doi.org/10.1007/s00264-003-0430-4
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DOI: https://doi.org/10.1007/s00264-003-0430-4