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Monoclonal antibody A7 coupled to magnetic particles as a contrast enhancing agent for magnetic resonance imaging of human colorectal carcinoma

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Abstract

Background: Local recurrence, the most frequent pattern of recurrence of rectal carcinoma, is almost always fatal. The difficulty of diagnosing local recurrence contributes importantly to the poor prognosis. Methods: We coupled monoclonal antibody (Mab) A7, which reacts specifically with human colorectal carcinoma, to ferromagnetic lignosite (FML) particles to distinguish rectal carcinoma from other tissues by magnetic resonance (MR) imaging. We examined retention of immunoreactivity by the A7-FML complexes in vitro, and also their distribution in vivo according to radiolabeling and MR imaging when injected into nude mice bearing human colorectal carcinoma xenografts. Results: A7-FML retained binding activity nearly identical to that of Mab A7. Significantly more 125I-labeled A7-FML accumulated in engrafted tumors than did 125I-labeled normal mouse IgG-FML complexes (P<0.05). A7-FML disappeared rapidly from the blood. Normal tissues accumulated less 125I-labeled A7-FML than tumors; this accumulation decreased linearly with time. In MR imaging, signal intensity was reduced in the tumor by the injection of A7-FML. Conclusions: A7-FML is potentially useful as a MR contrast enhancing agent for human colorectal carcinoma xenografts implanted subcutaneously.

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Acknowledgements

This work was supported by Foundation for Promotion of Cancer Research in Japan.

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Authors and Affiliations

  1. Department of Surgery, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji Kamigyo-ku, Kyoto, 602-8566, Japan

    Eigo Otsuji, Yoshiaki Kuriu, Kazuma Okamoto, Daisuke Ichikawa, Akeo Hagiwara & Hisakazu Yamagishi

  2. Department of Radiology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji Kamigyo-ku, Kyoto, 602-8566, Japan

    Hirotoshi Ito & Tsunehiko Nishimura

Authors
  1. Eigo Otsuji
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  2. Yoshiaki Kuriu
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  3. Kazuma Okamoto
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  4. Daisuke Ichikawa
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  5. Akeo Hagiwara
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  6. Hirotoshi Ito
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  7. Tsunehiko Nishimura
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  8. Hisakazu Yamagishi
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Correspondence to Eigo Otsuji.

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Otsuji, E., Kuriu, Y., Okamoto, K. et al. Monoclonal antibody A7 coupled to magnetic particles as a contrast enhancing agent for magnetic resonance imaging of human colorectal carcinoma. Cancer Immunol Immunother 55, 728–733 (2006). https://doi.org/10.1007/s00262-005-0067-9

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  • DOI: https://doi.org/10.1007/s00262-005-0067-9

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