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Class I myosin mediated endocytosis and polarization growth is essential for pathogenicity of Magnaporthe oryzae

  • Applied Genetics and Molecular Biotechnology
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Abstract

In eukaryotes, myosin provides the necessary impetus for a series of physiological processes, including organelle movement, cytoplasmic flow, cell division, and mitosis. Previously, three members of myosin were identified in Magnaporthe oryzae, with class II and class V myosins playing important roles in intracellular transport, fungal growth, and pathogenicity. However, limited is known about the biological function of the class I myosin protein in the rice blast fungus. Here, we found that Momyo1 is highly expressed during conidiation and infection. Functional characterization of this gene via RNA interference (RNAi) revealed that Momyo1 is required for vegetative growth, conidiation, melanin pigmentation, and pathogenicity of M. oryzae. The Momyo1 knockdown mutant is defective in formation of appressorium-like structures (ALS) at the hyphal tips. In addition, Momyo1 also displays defects on cell wall integrity, hyphal hydrophobicity, extracellular enzyme activities, endocytosis, and formation of the Spitzenkörper. Furthermore, Momyo1 was identified to physically interact with the MoShe4, a She4p/Dim1p orthologue potentially involved in endocytosis, polarization of the actin cytoskeleton. Overall, our findings provide a novel insight into the regulatory mechanism of Momyo1 that is involved in fungal growth, cell wall integrity, endocytosis, and virulence of M. oryzae.

Key points

• Momyo1 is required for vegetative growth and pigmentation of M. oryzae.

• Momyo1 is essential for cell wall integrity and endocytosis of M. oryzae.

• Momyo1 is involved in hyphal surface hydrophobicity of M. oryzae.

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Data availability

All data analyzed during this study are included in this published article (and its supplementary information files).

This article does not contain any studies with human participants or animals performed by any of the authors.

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Funding

This work was supported by the Foundation for the Author of National Excellent Doctoral Dissertation of PR China (Grant No: 201470 to MG), the National Natural Science Foundations of China (Grant No: 31671976 to MG), the Foundation for the Excellent Talents of Anhui Agricultural University (Grant No: RC2015002 to MG), and the Anhui Agricultural University Postgraduate Innovation Foundation (Grant No. 2020ysj-8 to ZCC).

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  1. Chengcheng Zheng and Weiwei Zhang have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, Anhui Agricultural University, 130 West of Changjiang Road, Hefei, 230036, China

    Chengcheng Zheng, Weiwei Zhang, Shulin Zhang, Guogen Yang, Leyong Tan & Min Guo

  2. Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei, 230036, China

    Chengcheng Zheng, Weiwei Zhang, Shulin Zhang, Guogen Yang, Leyong Tan & Min Guo

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  1. Chengcheng Zheng
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Contributions

CCZ measured phenotypes, analyzed the data, and wrote the manuscript. ZWW measured phenotypes, constructed strains for Co-Ip, revised the manuscript. SLZ help to write the manuscript. GGY participated in the writing and modification of the manuscript. LYT conducted construction of RNAi mutants, preparation of experimental materials, analyzation of the data. MG conceived and designed the experiments, analyzed the data, and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Min Guo.

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Zheng, C., Zhang, W., Zhang, S. et al. Class I myosin mediated endocytosis and polarization growth is essential for pathogenicity of Magnaporthe oryzae. Appl Microbiol Biotechnol 105, 7395–7410 (2021). https://doi.org/10.1007/s00253-021-11573-8

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