Abstract
The objective of this study was to develop biodegradable coatings for agriculture crop seeds based on starch, gelatin, and polyvinyl alcohol (PVA). Developed materials were characterized according to their microstructures, barrier properties, influence on germination of maize seeds, and ability to sustain Azospirillum brasilense Ab-V5 viability in coated maize seeds. The coatings were obtained employing different proportions of starch, gelatin, and PVA, ranging from 0 to 3.0 g/100 g of each material, respectively. Samples formulated with the pure polymers showed the highest values of water absorption capacity, solubility, and water vapor permeability, and the ternary mixtures showed the lowest values. Single polymer formulations and the binary starch-gelatin mixture (CS50GL50) favored maize seeds germination compared to the uncoated maize seeds. In addition, seed coating obtained from CS50GL50 formulation resulted in A. brasilense Ab-V5 viability in coated seeds up to 15 days after bacterial immobilization, being considered a promising low-cost, biodegradable, and renewable source material to be used in agriculture.
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References
Accinelli C, Abbas HK, Little NS, Kotowicz JK, Shier WT (2018) Biological control of aflatoxin production in corn using non-aflatoxigenic Aspergillus flavus administered as a bioplastic-based seed coating. Crop Prot 107:87–92. https://doi.org/10.1016/j.cropro.2018.02.004
Almeida C, Rocha SCS, Razer LF (2005) Polymer coating, germination and vigor of broccoli seeds. Sci Agric 62:221–226. https://doi.org/10.1590/S0103-90162005000300004
American Society for Testing and Materials – ASTM (2000) Standard test method for water vapor transmission of material – E96-00. Annual book of ASTM Standards. ASTM, Philadelphia
Avelar SAG, Sousa FV, Fiss G, Baudet L, Peske ST (2012) The use of film coating on the performance of treated corn seed. Rev Bras Sem 34:186–192
Bashan Y, de-Bashan LE, Prabhu SR (2016) Superior polymeric formulations and emerging innovative products of bacterial inoculants for sustainable agriculture and the environment. In: Singh H, Sarma B, Keswani C (eds) Agriculturally important microorganisms. Springer, Singapore, pp 15–46
Bertuzzi MA, Armada M, Gottifredi JC (2007a) Physicochemical characterization of starch based films. J Food Eng 82:17–25. https://doi.org/10.1016/j.jfoodeng.2006.12.016
Bertuzzi MA, Castro Vidaurre EF, Armada M, Gottifredi JC (2007b) Water vapor permeability of edible starch based films. J Food Eng 80:972–978. https://doi.org/10.1016/j.jfoodeng.2006.07.016
Brasil (2009) Regras para análise de sementes. Brasília: Ministério da Agricultura, Pecuária e Abastecimento, 2009. 399p
Girardi NS, Passone MA, García D, Nesci A, Etcheverry M (2018) Microencapsulation of Peumus boldus essential oil and its impact on peanut seed quality preservation. Ind Crop Prod 114:108–114. https://doi.org/10.1016/j.indcrop.2018.01.036.
Gontard N, Guilbert S, Cuq JL (1992) Edible wheat gluten films: influence of the main process variables on film properties using response surface methodology. J Food Sci 57:190–195
Gorim L, Asch F (2012) Effects of composition and share of seed coatings on the mobilization efficiency of cereal seeds during germination. J Agron Crop Sci 198:81–91. https://doi.org/10.1111/j.1439-037X.2011.00490.x
Hu J, Guan Y (2018) The application of high polymer materials in the aspects of seed technology In: Thakur V, Thakur M, Voicu S (eds) Polymer gels. Gels horizons: from science to smart materials, Springer, Singapore, pp 55–69.
Hungria M, Campo RJ, Souza EM, Pedrosa FO (2010) Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant Soil 331:413–425. https://doi.org/10.1007/s11104-009-0262-0
Jayasekara R, Harding I, Bowater I, Christie GB, Lonergan G (2004) Preparation, surface modification and characterization of solution cast starch PVA blended films. Polym Test 23:17–27. https://doi.org/10.1016/S0142-9418(03)00049-7
Jisha KC, Vijayakumari K, Puthur JT (2013) Seed priming for abiotic stress tolerance: an overview. Acta Physiol Plant 35:1381–1396. https://doi.org/10.1007/s11738-012-1186-5
Kashyap PL, Xiang X, Heiden P (2015) Chitosan nanoparticle based delivery systems for sustainable agriculture. Int J Biol Macromol 77:36–51. https://doi.org/10.1016/j.ijbiomac.2015.02.039
Marcelino PRF, Milani KML, Mali S, Santos OJAP, Oliveira ALM (2016) Formulations of polymeric biodegradable low-cost foam by melt extrusion to deliver plant growth-promoting bacteria in agricultural systems. Appl Microbiol Biotechnol 100:7323–7338. https://doi.org/10.1007/s00253-016-7566-9
Miles AA, Misra SS, Irwin JO (1938) The estimation of bactericidal power of the blood. J Hyg 38:732–749
Montesano FF, Parente A, Santamaria P, Sannino A, Serio F (2015) Biodegradable superabsorbent hydrogel increases water retention properties of growing media and plant growth. Agri Agri Sci Procedia 4:451–458. https://doi.org/10.1016/j.aaspro.2015.03.052
Müller CMO, Yamashita F, Laurindo JB (2008) Evaluation of the effects of glycerol and sorbitol concentration and water activity on the water barrier properties of cassava starch films through a solubility approach. Carbohydr Polym 72:82–87. https://doi.org/10.1016/j.carbpol.2007.07.026
Oliveira AF, Soldi V, Coelho CMM, Miqueloto A, Coimbra JLM (2009) Preparação, caracterização e propriedades de filmes poliméricos com potencial aplicação no recobrimento de sementes. Quim Nova 32:1845–1849. https://doi.org/10.1590/S0100-40422009000700030
Oliveira ALM, Santos OJAP, Marcelino PRF, Milani KML, Zuluaga MYA, Zucareli C, Gonçalves LSA (2017) Maize inoculation with Azospirillum brasilense Ab-V5 cells enriched with exopolysaccharides and polyhydroxybutyrate results in high productivity under low N fertilizer input. Front Microbiol 8:1–18. https://doi.org/10.3389/fmicb.2017.01873
Pal K, Banthia AK, Majumdar DK (2007) Preparation and characterization of polyvinyl alcohol-gelatin hydrogel membranes for biomedical applications. AAPS PharmSciTech 8:E142–E146. https://doi.org/10.1208/pt080121
Pedrini S, Merritt DJ, Stevens J, Dixon K (2017) Seed coating: science or marketing spin ? Trends Plant Sci 22:106–116. https://doi.org/10.1016/j.tplants.2016.11.002
Pizzoli APO, Yamashita F, Gonçalves OH, Shirai MA, Leimann FV (2017) The effect of gelatin amount on the properties of PLA/TPS/gelatin extruded sheets. Polimeros 27:27–34. https://doi.org/10.1590/0104-1428.2181
Santos RAL, Müller CMO, Grossmann MVE, Mali S, Yamashita F (2014) Starch/poly (butylene adipate–co-terephthalate)/montmorillonite films produced by blow extrusion. Quim Nova 37:937–942. https://doi.org/10.5935/0100-4042.20140170.
Schoebitz M, López MD, Roldán A (2013) Bioencapsulation of microbial inoculants for better soil-plant fertilization. A review. Agron Sustain Dev 33:751–765. https://doi.org/10.1007/s13593-013-0142-0
Sharma KK, Singh US, Sharma P, Kumar A, Sharma L (2015) Seed treatments for sustainable agriculture-a review. JANS 7:521–539
Tanada-Palmu PS, Proença PSP, Trani PE, Passos FA, Grosso CRF (2005) Recobrimento de sementes de brócolos e salsa com coberturas e filmes biodegradáveis. Bragantia 4:291–297. https://doi.org/10.1590/S0006-87052005000200016
Tang X, Alavi S (2011) Recent advances in starch, polyvinyl alcohol based polymer blends, nanocomposites and their biodegradability. Carbohydr Polym 85:7–16. https://doi.org/10.1016/j.carbpol.2011.01.030
Tao J, Zhang W, Liang L, Lei Z (2018) Effects of eco-friendly superabsorbent polymers on seed germination and seedling growth of maize. R Soc Open Sci 5:171184. https://doi.org/10.1098/rsos.171184
Tian H, Yan J, Rajulu AV, Xiang A, Luo X (2017) Fabrication and properties of polyvinyl alcohol/starch blend films: effect of composition and humidity. Int J Biol Macromol 96:518–523. https://doi.org/10.1016/j.ijbiomac.2016.12.067
Tomlinson I (2013) Doubling food production to feed the 9 billion: a critical perspective on a key discourse of food security in the UK. J Rural Stud 29:81–90. https://doi.org/10.1016/j.jrurstud.2011.09.001
Wang W, Wang K, Xiao J, Liu Y, Zhao Y, Liu A (2017) Performance of high amylose starch-composited gelatin films influenced by gelatinization and concentration. Int J Biol Macromol 94:258–265. https://doi.org/10.1016/j.ijbiomac.2016.10.014
Wu Z, Yao L, Kaleem I, Li C (2012) Application efficacy of biological seed coating agent from combination of PGPR on cotton in the field. In: Zhu E, Sambath S (eds) Information technology and agricultural engineering. Advances in intelligent and soft computing. Springer, Berlin, pp 903–910
Ziani K, Ursúa B, Maté JI (2010) Application of bioactive coatings based on chitosan for artichoke seed protection. Crop Prot 29:853–859. https://doi.org/10.1016/j.cropro.2010.03.002
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The authors wish to thank the Laboratory of Spectroscopy (ESPEC) and Laboratory of Electronic Microscopy and Microanalysis (LMEM) of the State University of Londrina.
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Vercelheze, A.E.S., Marim, B.M., Oliveira, A.L.M. et al. Development of biodegradable coatings for maize seeds and their application for Azospirillum brasilense immobilization. Appl Microbiol Biotechnol 103, 2193–2203 (2019). https://doi.org/10.1007/s00253-019-09646-w
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DOI: https://doi.org/10.1007/s00253-019-09646-w