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vB_LspM-01: a novel myovirus displaying pseudolysogeny in Lysinibacillus sphaericus C3-41

  • Applied microbial and cell physiology
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Abstract

Lysinibacillus sphaericus has great application potential not only in the biocontrol of mosquitoes but also in the bioremediation of toxic metals. Phages contribute to the genetic diversity and niche adaptation of bacteria, playing essential roles in their life cycle, but may also cause economic damage for industrially important bacteria through phage contamination during fermentation. In this study, the L. sphaericus phage vB_LspM-01, which belongs to the Myoviridae family, was isolated and characterized. Results showed that vB_LspM-01 could specifically infect most tested L. sphaericus isolates but was not active against isolates belonging to other species. Furthermore, phage-born endolysin exhibited a broader antimicrobial spectrum than the host range of the phage. The vB_LspM-01 genome had no obvious similarity with that of its host, and ca. 22.6% of putative ORFs could not get a match with the public databases. Phylogenic analysis based on the putative terminase large subunit showed high similarity with the phages identified with pac-type headful packaging. The vB_LspM-01 encoding genes were only detected in a tiny percentage of L. sphaericus C3-41 individual cells in the wild population, whereas they showed much higher frequency in the resistant population grown within the plaques; however, the phage genes could not be stably inherited during host cell division. Additionally, the vB_LspM-01 encoding genes were only detected in the host population during the logarithmic growth phase. The mitomycin C induction helped the propagation and lysogeny-lysis switch of vB_LspM-01. The study demonstrated that vB_LspM-01 can be present in a pseudolysogenic state in L. sphaericus C3-41 populations.

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Acknowledgments

We would like to thank Pei Zhang from the Core Facility and Technical Support, Wuhan Institute of Virology, for her help with producing EM micrographs.

Funding

This study was funded by the National Key R&D Programs of China (2016YFC1201000) and the National Natural Science Foundation of China (grant 31570007).

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Authors and Affiliations

  1. Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Peiling Geng, Xiaofu Wan, Jiao Cheng, Zhiming Yuan & Xiaomin Hu

  2. University of the Chinese Academy of Sciences, Beijing, 100039, China

    Peiling Geng, Xiaofu Wan & Jiao Cheng

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  1. Peiling Geng
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  2. Xiaofu Wan
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  3. Jiao Cheng
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Correspondence to Zhiming Yuan or Xiaomin Hu.

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Geng, P., Wan, X., Cheng, J. et al. vB_LspM-01: a novel myovirus displaying pseudolysogeny in Lysinibacillus sphaericus C3-41. Appl Microbiol Biotechnol 102, 10691–10702 (2018). https://doi.org/10.1007/s00253-018-9424-4

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