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Electrochemical immunosensors for Salmonella detection in food

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Abstract

Pathogen detection is a critical point for the identification and the prevention of problems related to food safety. Failures at detecting contaminations in food may cause outbreaks with drastic consequences to public health. In spite of the real need for obtaining analytical results in the shortest time possible, conventional methods may take several days to produce a diagnosis. Salmonella spp. is the major cause of foodborne diseases worldwide and its absence is a requirement of the health authorities. Biosensors are bioelectronic devices, comprising bioreceptor molecules and transducer elements, able to detect analytes (chemical and/or biological species) rapidly and quantitatively. Electrochemical immunosensors use antibody molecules as bioreceptors and an electrochemical transducer. These devices have been widely used for pathogen detection at low cost. There are four main techniques for electrochemical immunosensors: amperometric, impedimetric, conductometric, and potentiometric. Almost all types of immunosensors are applicable to Salmonella detection. This article reviews the developments and the applications of electrochemical immunosensors for Salmonella detection, particularly the advantages of each specific technique. Immunosensors serve as exciting alternatives to conventional methods, allowing “real-time” and multiple analyses that are essential characteristics for pathogen detection and much desired in health and safety control in the food industry.

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References

  • Afonso A, Perez-Lopes B, Faria RC, Mattoso L, Hernandez-Herrero M, Roig-Sagues A, Maltez-Da CM, Merkoci A (2013) Electrochemical detection of Salmonella using gold nanoparticles. Biosens Bioelectron 40:121–126

    Article  CAS  PubMed  Google Scholar 

  • Alonso-Lomillo MA, Domínguez-Renedo O, Arcos-Martínez MJ (2010) Screen-printed biosensors in microbiology; a review. Talanta 82:1629–1636

    Article  CAS  PubMed  Google Scholar 

  • Anandan V, Gangadharan R, Zhang G (2009) Role of SAM chain length in enhancing the sensitivity of nanopillar modified electrodes for glucose detection. Sensors 9:1295–1305

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Andrews WH, Jacobson A, Hammack T (2015) Food and Drug Administration. Bacteriological Analytical Manual (BAM). Chapter 5 Salmonella Available at: http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070149. htm (accessed 06.01.16)

  • Arora P, Sindhu A, Dilbaghi N, Chaudhury A (2011) Biosensors as innovative tools for the detection of food borne pathogens. Biosens Bioelectron 28:1–12

    Article  CAS  PubMed  Google Scholar 

  • Babacan S, Pirarnik P, Letcher S, Rand AG (2000) Evaluation of antibody immobilization methods for piezoelectric biosensor application. Biosens Bioelectron 15:615–621

    Article  CAS  PubMed  Google Scholar 

  • Bally M, Voros J (2009) Nanoscale labels: nanoparticles and liposomes in the development of high-performance biosensors. Nanomed 4:447–467

    Article  CAS  Google Scholar 

  • Brandão D, Liébana S, Campoy S, Cortés P, Alegret S, Pividori MI (2013) Electrochemical magneto-immunosensing of Salmonella based on nano and micro-sized magnetic particles. J Phys: Conf Series 421(012020):1–7

    Google Scholar 

  • Brzeska M, Panhorst M, Kamp PB, Schotter J, Reiss G, Pühler A, Becker A, Brückl H (2004) Detection and manipulation of biomolecules by magnetic carriers. J Biotechnol 112:25–33

    Article  CAS  PubMed  Google Scholar 

  • Burcu BE, Kemal SM (2015) Applications of electrochemical immunosensors for early clinical diagnostics. Talanta 132:162–174

    Article  Google Scholar 

  • Canbaz MÇ, Sezgintürk MK (2014) Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection. Anal Biochem 446:9–18

    Article  CAS  PubMed  Google Scholar 

  • Cao Y, Sun X, Guo Y, Zhao W, Wang X (2015) An electrochemical immunosensor based on interdigitated array microelectrode for the detection of chlorpyrifos. Bioprocess Biosyst Eng 38:307–313

    Article  CAS  PubMed  Google Scholar 

  • Centers for Disease Control and Prevention (2012) Pathogens causing US foodborne illnesses, hospitalizations, and deaths, 2000–2008 http://www.cdc.gov/foodborneburden/PDFs/pathogens-complete-list-01-12.pdf. Accessed 10 December 2015

  • Chumyim P, Rijiravanich P, Somasundrum M, Surareungchai W (2014) Detection of Salmonella enterica serovar Typhimurium in milk sample using electrochemical immunoassay and enzyme amplified labeling. Int Conf Agric Environ Biol Sci (AEBS-2014) Phuket (Thailand) 24–25

  • Clark LC Jr, Lyons C (1962) Electrode systems for continuous monitoring in cardiovascular surgery. Am NY Acad Sci 31:29–45

    Google Scholar 

  • Coen MC, Lehmann R, Groning P, Bielmann M, Galli C, Schlapbach L (2001) Adsorption and bioactivity of protein A on silicon surfaces studies by AFM and XPS. J Colloid Interf Sci 233:180–189

    Article  CAS  Google Scholar 

  • Croci L, Delibato E, Volpe G, Palleschi G (2001) A rapid electro-chemical ELISA for the detection of Salmonella in meat samples. Anal Lett 34:2597–2607

    Article  CAS  Google Scholar 

  • Delibato E, Volpe G, Stangalini D, De Medici D, Moscone D, Palleschi G (2006) Development of SYBR-green real-time PCR and a multichannel electrochemical immunosensor for specific detection of Salmonella enterica. Anal Lett 39:1611–1625

    Article  CAS  Google Scholar 

  • Derkus B, Kc E, Mazi H, Emregul E, Yumak T, Sinag A (2014) Protein a immunosensor for the detection of immunoglobulin G by impedance spectroscopy. Bioprocess Biosyst Eng 37:965–976

    Article  CAS  PubMed  Google Scholar 

  • Dev Das R, Roychaudhuri C, Maju S, Das S, Saha H (2009) Macroporous silicone based simple and eficiente trapping platform for electrical detection of Salmonella Typhimurium pathogens. Biosens Bioelectron 24:3215–3222

    Article  PubMed  Google Scholar 

  • Dill K, Stanker LH, Young CR (1999) Detection of Salmonella in poultry using a silicon chip-based biosensor. J Biochem Biophys Methods 41:61–67

    Article  CAS  PubMed  Google Scholar 

  • Dominguez-Benetton X, Srikanth S, Satyawali Y, Vanbroekhoven K, Pant D (2013) Enzymatic electrosynthesis: an overview on the progress in enzyme-electrodes for the production of electricity, fuels and chemicals. J Microb Biochem Technol S6:007. doi:10.4172/1948-5948. S6-007

    Google Scholar 

  • Dong J, Xu M, Maa Q, Ai S, Zhao H (2013) A label-free electrochemical impedance immunosensor based on AuNPs/PAMAM-MWCNT-Chi nanocomposite modified glassy carbon electrode for detection of Salmonella Typhimurium in milk. Food Chem 141:1980–1986

    Article  CAS  PubMed  Google Scholar 

  • European Food Safety Agency (2014) EFSA explains zoonotic diseases—Salmonella. http://www.efsa.europa.eu/en/search/doc/factsheetsalmonella.pdf. Accessed 20 October 2015

  • Feng P (2010) Rapid methods for detecting foodborne pathogens. In: Food and Drug Administration. Bacteriological Analytical Manual (BAM). Appendix 1. Available at: http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070149.htm (accessed 06.12.14)

  • Ferreira NS, Sales MGF (2014) Disposable immunosensor using a simple method for oriented antibody immobilization for label free real time detection of an oxidative stress biomarker implicated in cancer diseases. Biosens Bioelectron 53:193–199

    Article  CAS  PubMed  Google Scholar 

  • Food and Drug Administration (2012) Bad bug book, foodborne pathogenic microorganisms and natural toxins. Second edition. [Salmonella species, pp. 9–13]

  • Freitas M, Viswanathan S, Nouws H, Oliveira M, Delerue-Matos C (2014) Iron oxide/gold core/shell nanomagnetic probes and CdS biolabels for amplified electrochemical immunosensing of Salmonella Typhimurium. Biosens Bioelectron 51:195–200

    Article  CAS  PubMed  Google Scholar 

  • Gehring AG, Crawford CG, Mazenko RS, Van Houten LJ, Brewster JD (1996) Enzyme-linked immunomagnetic electrochemical detection of Salmonella Typhimurium. J Immunol Methods 195:15–25

    Article  CAS  PubMed  Google Scholar 

  • Gil ES, Mello GR (2010) Electrochemical biosensors in pharmaceutical analysis. Braz J Pharm Sci 46:375–391

    Article  CAS  Google Scholar 

  • Hayes JJ, Kennmore M, Badley A, Cullen DC (1998) AFM study of antibody adsorption to polystylene microtitre plates. Nanobiotechnol 4:141–151

    CAS  Google Scholar 

  • Holford TRJ, Davis F, Higson SPJ (2012) Recent trends in antibody based sensors. Biosens Bioelectron 34:12–24

    Article  CAS  PubMed  Google Scholar 

  • Hu CM, Dou W, Zhao G (2014) Enzyme immunosensor based on gold nanoparticles electroposition and streptavidin-biotin system for detection of S. Pullorum & S. Gallinarum. Electrochim Acta 117:239–245

  • Jaffrezic-Renault N, Dzyadevych SV (2008) Conductometric microbiosensors for environmental monitoring. Sens 8:2569–2588

    Article  Google Scholar 

  • Kaur J, Singh KV, Schmid AH, Varshney GC, Suri R, Raje M (2004) Atomic force spectrsocpy-based study of antibody pesticide interactions for characterization of immunosensor surface. Biosens Bioelectron 20:284–293

    Article  CAS  PubMed  Google Scholar 

  • Kim S, Choi SJ (2014) A lipid-based method for the preparation of a piezoelectric DNA biosensor. Anal Biochem 458:1–3

    Article  CAS  PubMed  Google Scholar 

  • Kim G-H, Rand AG, Letcher SV (2003) Impedance characterization of a piezoelectric immunosensor part II: Salmonella Typhimurium detection using magnetic enhancement. Biosens Bioelectron 18:91–99

    Article  CAS  PubMed  Google Scholar 

  • Kirsch J, Siltanen C, Zhou Q, Revzin A, Simonian A (2013) Biosensor technology: recent advances in threat agent detection and medicine. Chem Soc Rev 42:8733–8768

    Article  CAS  PubMed  Google Scholar 

  • Lawrence AJ, Moores GR (1972) Conductimetry in enzyme studies. European J Biochem 24:538–546

    Article  CAS  Google Scholar 

  • Lee W, Oh B-K, Bae YM, Paek S-H, Lee WH, Choi J-W (2003) Fabrication of self-assembled protein A monolayer and its application as an immunosensor. Biosens Bioelectron 19:185–192

    Article  CAS  PubMed  Google Scholar 

  • Lee K-M, Runyon M, Herrman TJ, Hsieh J, Phillips R (2015) Review of Salmonella detection and identification methods: aspects of rapid emergency response and food safety. Food Control 47:264–276

    Article  Google Scholar 

  • Liébana S, Lermo A, Alegret S, Pividori MI, Campoy S, Cortés MP (2009) Rapid detection of Salmonella in milk by electrochemical magneto-immunosensing. Biosens Bioelectron 25:510–513

    Article  PubMed  Google Scholar 

  • Liu Y, Che Y, Li Y (2001) Rapid detection of Salmonella Typhimurium using immunomagnetic separation and immuno-optical sensing method. Sens Actuators: B Chem 72:214–218

    Article  CAS  Google Scholar 

  • Liu X, Wang X, Zhang J, Feng H, Liu X, Wong DKY (2012) Detection of estradiol at an electrochemical immunosensor with a Cu UPD| DTBP-Protein G scaffold. Biosens Bioelectron 35:56–62

    Article  PubMed  Google Scholar 

  • Ma X, Jiang Y, Jia F, Chen J, Wang Z, Yu Y (2014) An aptamer-based electrochemical biosensor for the detection of Salmonella. J Microbiol Methods 98:94–98

    Article  CAS  PubMed  Google Scholar 

  • Mantzila AG, Maipa V, Prodromidis MI (2008) Development of a faradaic impedimetric immunosensor for the detection of Salmonella Typhimurium in milk. Anal Chem 80:1169–1175

    Article  CAS  PubMed  Google Scholar 

  • Margot H, Stephan R, Mahony E, Iversen C (2013) Comparison of rapid cultural methods for the detection of Salmonella species. International J Food Microbiol 163:47

    Article  CAS  Google Scholar 

  • Martín-Yerga D, González-García MB, Costa-García A (2013) Biosensor array based on the in situ detection of quantum dots as electrochemical label. Sens Actuators: B Chem 182:184–189

    Article  Google Scholar 

  • Meyer MHF, Hartmann M, Krause H-J, Blankenstein G, Mueller-Chorus B, Oster J, Miethe P, Keusgen M (2007) CRP determination based on a novel magnetic biosensor. Biosens Bioelectron 22:973–979

    Article  CAS  PubMed  Google Scholar 

  • Morales MD, Serra B, Guzmán-Vázquez de Prada A, Reviejo AJ, Pingarrón JM (2007) An electrochemical method for simultaneous detection and identification of Escherichia coli, Staphylococcus aureus and Salmonella Choleraesuis using a glucose oxidase-peroxidase composite biosensor. Analyst 132:572–578

    Article  CAS  PubMed  Google Scholar 

  • Mortari A, Lorenzelli L (2014) Recent sensing technologies for pathogen detection in milk: a review. Biosens Bioelectron 60:8–21

    Article  CAS  PubMed  Google Scholar 

  • Muhammad-Tahir Z, Alocilja EC (2003) A conductometric biosensor for biosecurity. Biosens Bioelectron 18:813–819

    Article  CAS  PubMed  Google Scholar 

  • Murugaiyan S, Ramasamy R, Gopal N (2014) Biosensors in clinical chemistry: an overview. Adv Biomed Res 3:67

    Article  PubMed  PubMed Central  Google Scholar 

  • Nandakumar S, Woolard SN, Yuan D, Rouse BT, Kumaraguru U (2008) Natural killer cells as novel helpers in anti-herpes simplex virus immune response. J Virol 82:10820–10831

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nandakumar V, Bishop D, Alonas E, Labelle J, Joshi L, Alford TI (2011) A low- cost electrochemical biosensor for rapid bacterial detection. IEEE Sensors J 11:210–216

    Article  CAS  Google Scholar 

  • Nguyen P-D, Tran TB, Nguyen DTX, Min J (2014) Magnetic silica nanotube-assisted impedimetric immunosensor for the separation and label-free detection of Salmonella Typhimurium. Sens Actuators: B. Chemical 197:314–320

    Article  CAS  Google Scholar 

  • Niraj MMGASP (2012) Histamine biosensor: a review. Int J Pharm Sci Res 3:4158–4168

    Google Scholar 

  • Olsen EV, Pathirana ST, Samoylov AM, Barbaree JM, Chin BA, Neely WC, Vodyanoy V (2003) Specific and selective biosensor for Salmonella and its detection in the environment. J Micorbiol Methods 53:273–285

    Article  CAS  Google Scholar 

  • Özel RE, Ispas C, Ganesana M, Leiter JC, Andreescu S (2014) Glutamate oxidase biosensor based on mixed ceria and titania nanoparticles for the detection of glutamate in hypoxic environments. Biosens Bioelectron 52:397–402

    Article  PubMed  Google Scholar 

  • Park I-S, Kim W-Y, Kim N (2000) Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp. Biosens Bioelectron 15:167–172

    Article  CAS  PubMed  Google Scholar 

  • Pathirana ST, Barbaree J, Chin BA, Hartell MG, Neely WC (2000) Rapid and sensitive biosensor for Salmonella. Biosens Bioelectron 15:135–144

    Article  CAS  PubMed  Google Scholar 

  • Pimenta-Martins MG, Furtado RF, Heneine LG, Dias RS, Morges MF, Alves CR (2012) Development of an amperometric immunosensor for detection of Staphylococcal enterotoxin type A in cheese. J Microbiol Methods 91:138–143

    Article  CAS  PubMed  Google Scholar 

  • Pohanka M, Skládal P (2008) Electrochemical biosensors—principles and applications. J Appl Biomed 6:57–64

    CAS  Google Scholar 

  • Pournaras AV, Koraki T, Prodromidis MI (2008) Development of an impedimetric immunosensor based on electropolymerized polytyramine films for the direct detection of Salmonella Typhimurium in pure cultures of type strains and inoculated real samples. Anal Chim Acta 624:301–307

    Article  CAS  PubMed  Google Scholar 

  • Prashar D (2012) Self assembled monolayers—a review. Int J Chem Tech Res 4:258–265

    CAS  Google Scholar 

  • Prodromidis MI (2010) Impedimetric immunosensors—a review. Electrochim Acta 55:4227–4233

    Article  CAS  Google Scholar 

  • Prusak-Sochaczewski E, Luong JTT, Guilbault GG (1990) Development of a piezoelectric immunosensor for the detection of Salmonella Typhimurium. Enzyme Microbiol Technol 12:173–177

    Article  CAS  Google Scholar 

  • Purvis D, Leonardova O, Farmakovsky D, Cherkasov V (2003). An ultrasensitive and stable potentiometric immunosensor. Biosens and Bioelectron 18:1385–1390

  • Qureshi A, Gurbuz Y, Niazi JH (2012) Biosensors for cardiac biomarkers detection: a review.(Report). Sens Actuators: B Chem 171:62–76

    Article  Google Scholar 

  • Ricci F, Adornetto G, Palleschi G (2012) A review of experimental aspects of electrochemical immunosensors. Electrochim Acta 84:74–83

    Article  CAS  Google Scholar 

  • Rickert J, Gopel W, Beck W, Jung G, Heiduschka P (1996) A ‘mixed’ self- assembled monolayer for an impedimetric immunosensor. Biosens Bioelectron 11:757–768

    Article  CAS  PubMed  Google Scholar 

  • Salam F, Tothill IE (2009) Detection of Salmonella Typhimurium using an electrochemical immunosensor. Biosens Bioelectron 24:2630–2636

    Article  CAS  PubMed  Google Scholar 

  • Saleem M (2013) Biosensors a promising future in measurements. IOP Conf. Series: Mater Sci Eng 51(012012):1–10

    Google Scholar 

  • Si S-H, Li X, Fung Y-S, Zhu D-R (2001) Rapid detection of Salmonella Enteritidis by piezoelectric immunosensor. Microchem J 68:21–27

    Article  CAS  Google Scholar 

  • Sibai A, Elamri K, Barbier D, Zaffrezic-Renault N, Souteyrand E (1996) Analysis of the polymer-antibody-antigen interaction in a capacitive immunosensor by FTIR difference spectroscopy. Sens actuators: B Chem 31:125–130

    Article  CAS  Google Scholar 

  • Singh R, Mukherjee MD, Sumana G, Gupta RK, Sood S, Malhotra BD (2014) Biosensors for pathogen detection: a smart approach towards clinical diagnosis. Sens actuators: B Chem 197:385–404

    Article  CAS  Google Scholar 

  • Skládal P, Kovar D, Krajicek V, Siskova P, Pribyl J, Svabenska E (2013) Electrochemical immunosensors for detection of microorganisms. Int J Electrochem Sci 8:1635–1649

    Google Scholar 

  • Soto AMG, Jaffari SA, Bone S (2001) Characterisation and optimization of AC conductometric biosensor. Biosens Bioelectron 16:23–29

    Article  Google Scholar 

  • Su L, Jia W, Hou C, Lei Y (2011) Microbial biosensors: a review. Biosens and Bioelectron 26:1788–1799

    Article  CAS  Google Scholar 

  • Vashist SK, Zheng D, Al-Rubeaan K, Luong JHT, Sheu F-S (2011) Advances in carbon nanotube based electrochemical sensors for bioanalytical applications. Biotechnol Adv 29:169–188

    Article  CAS  PubMed  Google Scholar 

  • Velusamy V, Arshak K, Korostynska O, Oliwa K, Adley C (2010) An overview of foodborne pathogen detection: in the perspective of biosensors. Biotechnol Adv 28:232–254

    Article  CAS  PubMed  Google Scholar 

  • Vidal JC, Bonel L, Ezquerra A, Hernandez S, Bertolin JR, Cubel C, Castillo JR (2013) Electrochemical affinity biosensors for detection of mycotoxins: a review. Biosens Bioelectron 49:146–158

    Article  CAS  PubMed  Google Scholar 

  • Vilarino N, Fonfria ES, Louzao MC, Botana LM (2009) Use of biosensors as alternatives to current regulatory methods for marine biotoxins. Sens 9(11):9414

    Article  CAS  Google Scholar 

  • Vo-Dihn T, Tromberg GD, Griffin KR, Ambrose MJ, Sepaniak MJ, Gardenhire EM (1987) Antibody-based fiber ptics biosensor for the carcinogen benzo (a) pyrene. Appl Spectro Sci 41:735–738

    Article  Google Scholar 

  • Wang J (2005) Nanomaterial based electrochemical biosensors. Analyst 130:421–426

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Ye Z, Ying Y (2012) New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria. Sensors 12:3449–3471

    Article  PubMed  PubMed Central  Google Scholar 

  • Wong YYNG, Ng MH, Si SH, Yao SZ, Fung YS (2002) Immunosensor for the differentiation and detection of Salmonella species based on quartz crystal microbalance. Biosens Bioelectron 17:676–684

    Article  CAS  PubMed  Google Scholar 

  • Yang G-J, Huang J-L, Meng W-J, Shen M, Jiao X-A (2009) A reusable capacitive immunosensor for detection of Salmonella spp. based on grafted ethylene diamine and self-assembled gold nanoparticle monolayers. Anal Chim Acta 647:159–166

    Article  CAS  PubMed  Google Scholar 

  • Zhao Y, Zheng Y, Kong R, Xia L, Qu F (2016) Ultrasensitive electrochemical immunosensor based on horseradish peroxidase (HRP)-loaded silica-poly (acrylic acid) brushes for protein biomarker detection. Biosens Bioelectron 75:383–388

    Article  CAS  PubMed  Google Scholar 

  • Zhu D, Yan Y, Lei P, Shen B, Cheng W, Ju H, Ding SJ (2014) A novel electrochemical sensing strategy for rapid and ultrasensitive detection of Salmonella by rolling circle amplification and DNA-AuNPs probe. Anal Chim Acta 846:44–50

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors acknowledge the financial support of the CNPq (Award number: 475174/2012-7) and CAPES Brazilian agencies. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Department of Chemistry, State University of Ceará, Av. Paranjana 1700, Fortaleza, 60.740-903, CE, Brazil

    Airis Maria Araújo Melo, Dalila L. Alexandre & Carlúcio R. Alves

  2. Embrapa Tropical Agroindustry, Rua Dra. Sara Mesquita 2270, Fortaleza, CE, 60.511-110, Brazil

    Roselayne F. Furtado & Maria F. Borges

  3. Department of Food Science and Technology, Federal University of Ceará, Campus do Pici, Bloco 858, Fortaleza, CE, Brazil

    Evânia Altina T. Figueiredo

  4. USDA Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 North University Street, Peoria, IL, 61604, USA

    Atanu Biswas

  5. USDA Agricultural Research Service, Southern Regional Research Center, 1100 Robert E. Lee Blvd, New Orleans, LA, 70124, USA

    Huai N. Cheng

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  1. Airis Maria Araújo Melo
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  2. Dalila L. Alexandre
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Correspondence to Airis Maria Araújo Melo.

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Melo, A.M.A., Alexandre, D.L., Furtado, R.F. et al. Electrochemical immunosensors for Salmonella detection in food. Appl Microbiol Biotechnol 100, 5301–5312 (2016). https://doi.org/10.1007/s00253-016-7548-y

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