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A novel heterotrophic nitrifying and aerobic denitrifying bacterium, Zobellella taiwanensis DN-7, can remove high-strength ammonium

  • Environmental biotechnology
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Abstract

A novel heterotrophic bacterium capable of heterotrophic nitrification and aerobic denitrification was isolated from ammonium contaminated landfill leachate and physiochemical and phylogenetically identified as Zobellella taiwanensis DN-7. DN-7 converted nitrate, nitrate, and ammonium to N2 as the primary end product. Single factor experiments suggested that the optimal conditions for ammonium removal were trisodium citrate as carbon source, C/N ratio 8, pH 8.0–10.0, salinity less than 3 %, temperature 30 °C, and rotation speed more than 150 rpm. Specifically, DN-7 could remove 1000.0 and 2000.0 mg/L NH4 +-N completely within 96 and 216 h, with maximum removal rates of 19.6 and 17.3 mg L−1 h−1, respectively. These results demonstrated that DN-7 is a promising candidate for application of high-strength ammonium wastewater treatments.

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Acknowledgments

We thank the Natural Science Foundation of China (Grant No. 51578528) and Natural Science Foundation of Chongqing (Grant No. cstc2014jcyjA20006) for their financial support.

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Authors and Affiliations

  1. Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, Chongqing, 400714, China

    Yu Lei, Yangqing Wang, Hongjie Liu & Liyan Song

  2. Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI, 48109, USA

    Chuanwu Xi

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  1. Yu Lei
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  2. Yangqing Wang
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  3. Hongjie Liu
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  4. Chuanwu Xi
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  5. Liyan Song
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Correspondence to Liyan Song.

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Lei, Y., Wang, Y., Liu, H. et al. A novel heterotrophic nitrifying and aerobic denitrifying bacterium, Zobellella taiwanensis DN-7, can remove high-strength ammonium. Appl Microbiol Biotechnol 100, 4219–4229 (2016). https://doi.org/10.1007/s00253-016-7290-5

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