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Isolation of a novel beta-cypermethrin degrading strain Bacillus subtilis BSF01 and its biodegradation pathway

  • Environmental biotechnology
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Abstract

Continuous use of the pyrethroid insecticide beta-cypermethrin (beta-cp) has resulted in serious environmental contamination problems. We report here that a novel bacterial strain BSF01, which was isolated from activated sludge and identified as Bacillus subtilis (collection number: CCTCC AB 2014103), showed high efficiency in degrading beta-cp. Strain BSF01 was able to utilize beta-cp as the sole carbon source for growth and degraded 89.4 % of 50 mg L−1 beta-cp within 7 days. The optimal conditions for beta-cp degradation were determined to be 34.5 °C, pH 6.7, and inocula amount 0.11 g dry wt L−1 using response surface methodology. The kinetic parameters q max, K s, and K i were established to be 2.19 day−1, 76.37 mg L−1, and 54.14 mg L−1, respectively. The critical inhibitor concentration was determined to be 64.30 mg L−1. Seven metabolites were identified by gas chromatography–mass spectrometry. Furthermore, a novel biodegradation pathway for beta-cp was proposed on the basis of analysis of the metabolites. This strain was also capable of degrading a wide range of pyrethroid insecticides including cypermethrin, deltamethrin, cyhalothrin, and beta-cyfluthrin, which similar to beta-cp are hazardous chemicals. Taken together, our results depict the biodegradation pathway of beta-cp and highlight the promising potentials of strain BSF01 in bioremediation of pyrethroid-contaminated environments.

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Acknowledgments

We would like to thank Dr. Jingjing Liu for her kind help. The study was funded by the National Natural Science Foundation (30871660 and 31371960), P.R. China.

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Authors and Affiliations

  1. Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Ying Xiao, Wei Hu, Meiying Hu & Guohua Zhong

  2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Shaohua Chen

  3. Guangdong Tianhe Agricultural Means of Production Co., Ltd., Guangzhou, 510642, People’s Republic of China

    Yuanqi Gao

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  1. Ying Xiao
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  2. Shaohua Chen
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  3. Yuanqi Gao
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  4. Wei Hu
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  5. Meiying Hu
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  6. Guohua Zhong
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Correspondence to Meiying Hu or Guohua Zhong.

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Xiao, Y., Chen, S., Gao, Y. et al. Isolation of a novel beta-cypermethrin degrading strain Bacillus subtilis BSF01 and its biodegradation pathway. Appl Microbiol Biotechnol 99, 2849–2859 (2015). https://doi.org/10.1007/s00253-014-6164-y

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  • DOI: https://doi.org/10.1007/s00253-014-6164-y

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