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Analysis of the biological activities of Saccharomyces cerevisiae expressing intracellular EGF, extracellular EGF, and tagged EGF in early-weaned rats

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Abstract

A growing number of studies suggest that epidermal growth factor (EGF) plays an important role in early-weaned animals. The objective of this experiment was to compare the biological activity of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-EGF) from Saccharomyces cerevisiae (S. cerevisiae) both in vivo and in vitro. Strains of S. cerevisiae expressing IE-EGF, EE-EGF, and T-EGF were designated INVSc1-IE(+), INVSc1-EE(+), and INVSc1-TE(−), respectively. The production performance, intestinal development, physio-biochemical indexes, and immunological function of early-weaned rats were measured in vivo to evaluate the biological activity of IE-EGF, EE-EGF, and T-EGF. In addition, the proliferation of rat enterocyte was also measured in vitro. In the in vivo experiment, the recombinant S. cerevisiae was shown to survive throughout the intestinal tract. The production performance (e.g., body weight) and intestinal development (e.g., mean villous height, crypt depth, total protein, DNA, and RNA) of the rats were significantly enhanced in the INVSc1-IE(+) group compared with the INVSc1-EE(+) and INVSc1-TE(−) groups (P < 0.05). However, the levels of lactate dehydrogenase (LDH), immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) showed no difference in the INVSc1-IE(+) group compared to the INVSc1-EE(+) and INVSc1-TE(−) groups (P > 0.05), with the only significant difference being found for creatine kinase (CK) (P < 0.05). In the in vitro experiment, the proliferation of enterocyte was significantly stimulated by both IE-EGF and EE-EGF compared with T-EGF (P < 0.05). Herein, IE-EGF is more suitable for application to early-weaned animals compared with EE-EGF and T-EGF.

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Acknowledgments

We thank the teachers and graduate students at the Key Laboratory of Animal Genetics and Breeding. This study was supported financially by the Ministry of Science and Technology of Sichuan Province (2012NZ0033).

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Authors and Affiliations

  1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu City, 610041, People’s Republic of China

    Shujin Wang, Huina Chen, Zhengfan Zhang, Yanlin Huang & Chunhua Guo

  2. Shenzhen Premixinve Nutrition Co., LTD, Shenzhen, 518103, People’s Republic of China

    Lin Zhou

  3. Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, People’s Republic of China

    Yangchun Cao

  4. Animal Science Research Institute of Sichuan Province, Chengdu, 610041, People’s Republic of China

    Jiabao Yang

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  1. Shujin Wang
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Correspondence to Chunhua Guo.

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Wang, S., Zhou, L., Chen, H. et al. Analysis of the biological activities of Saccharomyces cerevisiae expressing intracellular EGF, extracellular EGF, and tagged EGF in early-weaned rats. Appl Microbiol Biotechnol 99, 2179–2189 (2015). https://doi.org/10.1007/s00253-014-6044-5

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