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The first set of expressed sequence tags (EST) from the medicinal mushroom Agaricus subrufescens delivers resource for gene discovery and marker development

  • Applied genetics and molecular biotechnology
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Abstract

Agaricus subrufescens is one of the most important culinary-medicinal cultivable mushrooms with potentially high-added-value products and extended agronomical valorization. The development of A. subrufescens-related technologies is hampered by, among others, the lack of suitable molecular tools. Thus, this mushroom is considered as a genomic orphan species with a very limited number of available molecular markers or sequences. To fill this gap, this study reports the generation and analysis of the first set of expressed sequence tags (EST) for A. subrufescens. cDNA fragments obtained from young sporophores (SP) and vegetative mycelium in liquid culture (CL) were sequenced using 454 pyrosequencing technology. After assembly process, 4,989 and 5,125 sequences were obtained in SP and CL libraries, respectively. About 87 % of the EST had significant similarity with Agaricus bisporus-predicted proteins, and 79 % correspond to known proteins. Functional categorization according to Gene Ontology could be assigned to 49 % of the sequences. Some gene families potentially involved in bioactive compound biosynthesis could be identified. A total of 232 simple sequence repeats (SSRs) were identified, and a set of 40 EST-SSR polymorphic markers were successfully developed. This EST dataset provides a new resource for gene discovery and molecular marker development. It constitutes a solid basis for further genetic and genomic studies in A. subrufescens.

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Acknowledgments

This work was financially supported by a research project funded by a bilateral cooperation between Mexico (project 115790 CONACYT) and France (ANR-09-BLAN-0391-01). We acknowledge the URGI platform and particularly people involved in the GnpIS information system development and system administration.

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Authors and Affiliations

  1. INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d’Ornon, France

    Marie Foulongne-Oriol, Cathy Spataro, Nathalie Ferrer & Jean-Michel Savoie

  2. INRA, UR1164 URGI, Unité de Recherche Génomique-Info, Versailles, France

    Nicolas Lapalu & Joelle Amselem

  3. INRA, UR1290 BIOGER, Biologie et Gestion des Risques en Agriculture, Thiverval-Grignon, France

    Nicolas Lapalu & Joelle Amselem

  4. Department of Toxicology, UFR des Sciences Pharmaceutiques, Université Bordeaux Segalen, Bordeaux, France

    Cyril Férandon

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  1. Marie Foulongne-Oriol
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Correspondence to Marie Foulongne-Oriol.

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Joelle Amselem and Jean-Michel Savoie were joint senior authors on this work.

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Foulongne-Oriol, M., Lapalu, N., Férandon, C. et al. The first set of expressed sequence tags (EST) from the medicinal mushroom Agaricus subrufescens delivers resource for gene discovery and marker development. Appl Microbiol Biotechnol 98, 7879–7892 (2014). https://doi.org/10.1007/s00253-014-5844-y

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