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Identification of the specific electron transfer proteins, ferredoxin, and ferredoxin reductase, for CYP105D7 in Streptomyces avermitilis MA4680

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Abstract

It was previously proposed that regiospecific hydroxylation of daidzein at 3′-position is mediated by cytochrome P450 hydroxylase (CYP105D7) in the presence of putidaredoxin (CamB) and putidaredoxin reductase (CamA) as electron transfer proteins from Pseudomonas putida. The genome sequence of Streptomyces avermitilis MA4680 revealed 33 P450 (CYPs) with 6 ferredoxin reductases (Fprs) and 9 ferredoxins (Fdxs) as their putative electron transfer partner proteins. To identify right endogenous electron transfer proteins for CYP105D7 activity, in vitro reconstitution, gene disruption, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) mRNA expression profile analysis were examined. The most effective electron transfer proteins for CYP105D7 appear to be FdxH (SAV7470), which is located downstream to CYP105D7 as a cluster, and FprD (SAV5675). Throughout our overall analysis, we proposed that the primary electron transfer pathway for CYP105D7 follows as such NAD(P)H→FdxH→FprD→CYP105D7.

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Acknowledgments

This research was supported by WCU (World Class University) program through the National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (R322012000102130).

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Author notes

  1. Bishnu Prasad Pandey

    Present address: Department of Natural Science, School of Science, Kathmandu University, Dhulikhel, Kavre, Nepal

Authors and Affiliations

  1. School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Institute of Bioengineering, Seoul National University, Seoul, South Korea

    Bishnu Prasad Pandey, Nahum Lee, Kwon-Young Choi, Ji-Nu Kim, Eun-Jung Kim & Byung-Gee Kim

  2. Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea

    Byung-Gee Kim

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  1. Bishnu Prasad Pandey
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  2. Nahum Lee
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  3. Kwon-Young Choi
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  4. Ji-Nu Kim
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Correspondence to Byung-Gee Kim.

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Pandey, B.P., Lee, N., Choi, KY. et al. Identification of the specific electron transfer proteins, ferredoxin, and ferredoxin reductase, for CYP105D7 in Streptomyces avermitilis MA4680. Appl Microbiol Biotechnol 98, 5009–5017 (2014). https://doi.org/10.1007/s00253-014-5525-x

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  • DOI: https://doi.org/10.1007/s00253-014-5525-x

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