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Prophylactic and therapeutic efficacy of the epitope vaccine CTB-UA against Helicobacter pylori infection in a BALB/c mice model

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Abstract

Epitope vaccine based on the enzyme urease of Helicobacter pylori is a promising option for prophylactic and therapeutic vaccination against H. pylori infection. In our previous study, the epitope vaccine CTB-UA, which was composed of the mucosal adjuvant cholera toxin B subunit (CTB) and an epitope (UreA183–203) from the H. pylori urease A subunit (UreA) was constructed. This particular vaccine was shown to have good immunogenicity and immunoreactivity and could induce specific neutralizing antibodies, which exhibited effectively inhibitory effects on the enzymatic activity of H. pylori urease. In this study, the prophylactic and therapeutic efficacy of the epitope vaccine CTB-UA was evaluated in a BALB/c mice model. The experimental results indicated that oral prophylactic or therapeutic immunization with CTB-UA significantly decreased H. pylori colonization compared with oral immunization with PBS. The results also revealed that the protection was correlated with antigen-specific IgG, IgA, and mucosal secretory IgA antibody responses. CTB-UA may be a promising vaccine candidate for the control of H. pylori infection.

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Acknowledgments

This work was supported by the Science Foundation of China Pharmaceutical University (grant no. JKY2009023), Postgraduate Innovation Project of Jiangsu Province (grant no. CXZZ11_0817), and the National Major Special Program of New Drug Research and Development (grant no. 2012ZX09103-301-008). We especially thank Professor Wutong Wu for his contributions in the implementation of this experiment. Meanwhile, B.S Catherine J Tsang is thanked for the careful revision of this paper.

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Authors and Affiliations

  1. Biotechnology Center, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, 210009, China

    Le Guo, Kunmei Liu, Xiaokang Li, Jiajie Tu, Feng Tang, Yingying Xing & Tao Xi

  2. School of Laboratory Medicine, Ningxia Medical University, Yinchuan, 750004, China

    Le Guo & Guangxian Xu

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  1. Le Guo
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  2. Kunmei Liu
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  3. Guangxian Xu
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  4. Xiaokang Li
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  5. Jiajie Tu
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  6. Feng Tang
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  7. Yingying Xing
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  8. Tao Xi
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Correspondence to Tao Xi.

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Le Guo and Kunmei Liu contributed equally to this article.

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Guo, L., Liu, K., Xu, G. et al. Prophylactic and therapeutic efficacy of the epitope vaccine CTB-UA against Helicobacter pylori infection in a BALB/c mice model. Appl Microbiol Biotechnol 95, 1437–1444 (2012). https://doi.org/10.1007/s00253-012-4122-0

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  • DOI: https://doi.org/10.1007/s00253-012-4122-0

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