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Modification of the TRX2 gene dose in Saccharomyces cerevisiae affects hexokinase 2 gene regulation during wine yeast biomass production

  • Applied microbial and cell physiology
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Abstract

In the industrial yeast biomass production process, cells undergo an oxidative and other stresses which worsen the quality of the produced biomass. The overexpression of the thioredoxin codifying gene TRX2 in a wine Saccharomyces cerevisiae strain increases resistance to oxidative stress and industrial biomass production yield. We observed that variations in the TRX2 gene dose in wine yeast strains are relevant to determine the fermentative capacity throughout the industrial biomass production process. So, we studied the molecular changes using a transcriptomic approach under these conditions. The results provide an overview of the different metabolic pathways affected during industrial biomass production by TRX2 gene manipulation. The oxidative stress-related genes, like those related with the glutathione metabolism, presented outstanding variations. The data also allowed us to propose new thioredoxin targets in S. cerevisiae, such as hexokinase 2, with relevance for industrial fermentation performance.

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Acknowledgments

This work has been supported by grants AGL 2005-00508 and AGL 2008-00060 from the Spanish Ministry of Education and Science (MEC). R.G-P. was a predoctoral fellow of the I3P program from the CSIC (Spanish National Research Council). We thank the Proteomic and Transcriptomic Service of the Institute of Agrochemistry and Food Technology (IATA, CSIC) for their support in the transcriptomic analysis.

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  1. Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain

    Rocío Gómez-Pastor & Emilia Matallana

  2. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, Avda Agustín Escardino 7, 46980, Paterna, Valencia, Spain

    Roberto Pérez-Torrado & Emilia Matallana

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  1. Rocío Gómez-Pastor
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Correspondence to Emilia Matallana.

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Gómez-Pastor, R., Pérez-Torrado, R. & Matallana, E. Modification of the TRX2 gene dose in Saccharomyces cerevisiae affects hexokinase 2 gene regulation during wine yeast biomass production. Appl Microbiol Biotechnol 94, 773–787 (2012). https://doi.org/10.1007/s00253-011-3738-9

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  • DOI: https://doi.org/10.1007/s00253-011-3738-9

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