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Continuous production of isopropanol and butanol using Clostridium beijerinckii DSM 6423

  • Biotechnological Products and Process Engineering
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Abstract

Clostridium beijerinckii DSM 6423 was studied using different continuous production methods to give maximum and stable production of isopropanol and n-butanol. In a single-stage continuous culture, when wood pulp was added as a cell holding material, we could increase the solvent productivity from 0.47 to 5.52 g L−1 h−1 with the yield of 54% from glucose. The overall solvent concentration of 7.51 g L−1 (39.4% isopropanol and 60.6% n-butanol) with the maximum solvent productivity of 0.84 g L−1 h−1 was obtained with two-stage continuous culture. We were able to run the process for more than 48 overall retention times without losing the ability to produce solvents.

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Authors and Affiliations

  1. School of Chemical Technology, Department of Biotechnology and Chemical Technology, Aalto University, POB 16100, 00076, Aalto, Finland

    Shrikant A. Survase, German Jurgens & Tom Granström

  2. Department of Chemical and Biological Engineering, University of Maine, 5737 Jenness Hall, Orono, ME, 04469-5737, USA

    Adriaan van Heiningen

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  1. Shrikant A. Survase
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  2. German Jurgens
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  3. Adriaan van Heiningen
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  4. Tom Granström
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Correspondence to Tom Granström.

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Survase, S.A., Jurgens, G., van Heiningen, A. et al. Continuous production of isopropanol and butanol using Clostridium beijerinckii DSM 6423. Appl Microbiol Biotechnol 91, 1305–1313 (2011). https://doi.org/10.1007/s00253-011-3322-3

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  • DOI: https://doi.org/10.1007/s00253-011-3322-3

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