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Effects of hydroxypropyl-β-cyclodextrin on cell growth, activity, and integrity of steroid-transforming Arthrobacter simplex and Mycobacterium sp.

  • Applied Microbial and Cell Physiology
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Abstract

A comparative investigation was performed on the effects of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the growth, biocatalytic activity, and cell integrity of Arthrobacter simplex TCCC 11037 (ASP) and Mycobacterium sp. NRRL B-3683 (MSP). The addition of HP-β-CD to ASP medium improved its cell wall permeability for lipophilic compounds but significantly inhibited its growth and biocatalytic activity. On the other hand, the addition of HP-β-CD to MSP broth had lesser effects. Atomic force microscopy scanning analysis revealed that HP-β-CD damaged the cell integrity in ASP, especially the outermost cell surface structure, but not in MSP, which remained intact, owing to the differences in their cell wall and cell membrane composition. Protein leaking and lipid content in ASP increased with increased HP-β-CD concentration, indicating possible alterations in ASP cell membrane features caused by HP-β-CD. These alterations may also explain the slow cell growth and decreased cell ΔΨm in ASP upon the addition of HP-β-CD.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 20776111, 21076158), the Program for New Century Excellent Talents in University (No. NCET-08-0911), and the Foundation for Excellent Doctoral Dissertations of TianJin University of Science and Technology in 2010 (No. B201001).

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  1. Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Yanbing Shen, Min Wang, Liting Zhang, Yinhu Ma, Bing Ma, Yu Zheng, Hao Liu & Jianmei Luo

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  1. Yanbing Shen
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  2. Min Wang
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  3. Liting Zhang
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  4. Yinhu Ma
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  7. Hao Liu
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Correspondence to Min Wang.

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Shen, Y., Wang, M., Zhang, L. et al. Effects of hydroxypropyl-β-cyclodextrin on cell growth, activity, and integrity of steroid-transforming Arthrobacter simplex and Mycobacterium sp.. Appl Microbiol Biotechnol 90, 1995–2003 (2011). https://doi.org/10.1007/s00253-011-3214-6

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