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On-line infrared spectroscopy for bioprocess monitoring

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Abstract

One of the major aims of bioprocess engineering is the real-time monitoring of important process variables. This is the basis of precise process control and is essential for high productivity as well as the exact documentation of the overall production process. Infrared spectroscopy is a powerful analytical technique to analyze a wide variety of organic compounds. Thus, infrared sensors are ideal instruments for bioprocess monitoring. The sensors are non-invasive, have no time delay due to sensor response times, and have no influence on the bioprocess itself. No sampling is necessary, and several components can be analyzed simultaneously. In general, the direct monitoring of substrates, products, metabolites, as well as the biomass itself is possible. In this review article, insights are provided into the different applications of infrared spectroscopy for bioprocess monitoring and the complex data interpretation. Different analytical techniques are presented as well as example applications in different areas.

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Authors and Affiliations

  1. Institut für Technische Chemie, Leibniz Universität Hannover, Callinstr. 3, 30167, Hannover, Germany

    Daniel Landgrebe, Claas Haake, Tim Höpfner, Sascha Beutel, Bernd Hitzmann & Thomas Scheper

  2. Process Sciences & Production, Novartis Biologics, 4002, Basel, Switzerland

    Martin Rhiel

  3. Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, 80523-1370, USA

    Kenneth F. Reardon

Authors
  1. Daniel Landgrebe
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  2. Claas Haake
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  3. Tim Höpfner
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  4. Sascha Beutel
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  5. Bernd Hitzmann
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  6. Thomas Scheper
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  7. Martin Rhiel
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  8. Kenneth F. Reardon
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Correspondence to Thomas Scheper.

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Landgrebe, D., Haake, C., Höpfner, T. et al. On-line infrared spectroscopy for bioprocess monitoring. Appl Microbiol Biotechnol 88, 11–22 (2010). https://doi.org/10.1007/s00253-010-2743-8

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  • DOI: https://doi.org/10.1007/s00253-010-2743-8

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