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Effect of ultrafine-grained titanium surfaces on adhesion of bacteria

  • Applied Microbial and Cell Physiology
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Abstract

The influence of the ultrafine crystallinity of commercial purity grade 2 (as-received) titanium and titanium modified by equal channel angular pressing (modified titanium) on bacterial attachment was studied. A topographic profile analysis of the surface of the modified titanium revealed a complex morphology of the surface. Its prominent micro- and nano-scale features were 100–200-nm-scale undulations with 10–15 μm spacing. The undulating surfaces were nano-smooth, with height variations not exceeding 5–10 nm. These surface topography characteristics were distinctly different from those of the as-received samples, where broad valleys (up to 40–60 μm) were detected, whose inner surfaces exhibited asperities approximately 100 nm in height spaced at 1–2 μm. It was found that each of the three bacteria strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 68.5, Pseudomonas aeruginosa ATCC 9025 and Escherichia coli K12, responded differently to the two types of titanium surfaces. Extreme grain refinement by ECAP resulted in substantially increased numbers of cells attached to the surface compared to as-received titanium. This enhanced degree of attachment was accompanied with an increased level of extracellular polymeric substances (EPS) production by the bacteria.

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Acknowledgment

This study was supported in part by the Australian Research Council (ARC).

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Authors and Affiliations

  1. Faculty of Life and Social Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn, Victoria, 3122, Australia

    Vi Khanh Truong, Russell J. Crawford & Elena P. Ivanova

  2. Department of Materials Engineering, Monash University, Clayton, Victoria, 3800, Australia

    Stuart Rundell, Rimma Lapovok & Yuri Estrin

  3. ARC Centre of Excellence for Design in Light Metals, Clayton, Victoria, 3800, Australia

    Rimma Lapovok & Yuri Estrin

  4. IRIS, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn, Victoria, 3122, Australia

    James Y. Wang & Christopher C. Berndt

  5. Centre for Astrophysics and Supercomputing, Swinburne University of Technology, P.O. Box 218, Hawthorn, Victoria, 3122, Australia

    David G. Barnes & Christopher J. Fluke

Authors
  1. Vi Khanh Truong
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  2. Stuart Rundell
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  3. Rimma Lapovok
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  4. Yuri Estrin
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  5. James Y. Wang
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  6. Christopher C. Berndt
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  7. David G. Barnes
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  8. Christopher J. Fluke
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  9. Russell J. Crawford
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  10. Elena P. Ivanova
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Corresponding author

Correspondence to Elena P. Ivanova.

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ESM 1

Three-dimensional projections of CLSM images of representative S. aureus specimens on the as-received (left) and modified (right) titanium surfaces after 18-h incubation. Bacterial colonies (stained red with Rhodamine Red-X) produce EPS (stained green with concanavalin A Alexa 488) across the plate surface. Readers using version 8.0 or higher of Acrobat Reader can enable interactive, three-dimensional views of the data by clicking on the figure panels. Once enabled, 3-d mode allows the reader to rotate and zoom the view using the computer mouse (PDF 1.7 MB)

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Truong, V.K., Rundell, S., Lapovok, R. et al. Effect of ultrafine-grained titanium surfaces on adhesion of bacteria. Appl Microbiol Biotechnol 83, 925–937 (2009). https://doi.org/10.1007/s00253-009-1944-5

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  • DOI: https://doi.org/10.1007/s00253-009-1944-5

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