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Optimization of medium composition for the production of alkaline β-mannanase by alkaliphilic Bacillus sp. N16-5 using response surface methodology

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Abstract

In this work, a 22 factorial design was employed combining with response surface methodology (RSM) to optimize the medium compositions for the production of alkaline β-mannanase by alkaliphilic Bacillus sp. N16-5 isolated previously from sediment of Wudunur Soda Lake in Inner Mongolia, China. The central composite design (CCD) used for the analysis of treatment combinations showed that a second-order polynomial regression model was in good agreement with experimental results, with R 2 = 0.9829 (P < 0.05). The maximum activity was obtained at NaCl concentration (84.4 g l−1) and sodium glutamate (3.11 g l−1) and a high medium pH around 10.0. Under such conditions, the activity of alkaline β-mannanase achieved 310.1 U/ml in the scale of 5-l fermenter, which was increased nearly twice compared with the original. Through optimization, the substrates shifted from the expensive substrates, such as locust bean gum and peptone, to the inexpensive ones such as konjac powder, soymeal, and sodium glutamate. The experiment results also suggested that the environmental conditions of high salinity and high alkalinity, as well as the inducer substrates, play very important roles in the production of the alkaline β-mannanase by alkaliphilic Bacillus sp. N16-5.

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Acknowledgment

This work was supported by grants of National Basic Research Program of China (No.2007CB707804), National High-Tech Program (No. 2006AA020104), Jiangsu Planned Projects for Postdoctoral Research Funds (No.0601004A), and Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0532). The authors would thank Dr. Luhong Tang for his critical reading of this manuscript.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, 170 Huihe Road, Wuxi, 214036, People’s Republic of China

    Shan-shan Lin & Zheng-Hong Xu

  2. School of Biotechnology, Southern Yangtze University, Wuxi, 214036, People’s Republic of China

    Shan-shan Lin, Wen-fang Dou, Hong-yu Xu, Hua-zhong Li & Zheng-Hong Xu

  3. Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China

    Yan-he Ma

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  1. Shan-shan Lin
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  2. Wen-fang Dou
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  3. Hong-yu Xu
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  4. Hua-zhong Li
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  5. Zheng-Hong Xu
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Correspondence to Zheng-Hong Xu.

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Lin, Ss., Dou, Wf., Xu, Hy. et al. Optimization of medium composition for the production of alkaline β-mannanase by alkaliphilic Bacillus sp. N16-5 using response surface methodology. Appl Microbiol Biotechnol 75, 1015–1022 (2007). https://doi.org/10.1007/s00253-007-0907-y

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  • DOI: https://doi.org/10.1007/s00253-007-0907-y

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