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Biosynthesis of poly(ɛ-l-lysine)s in two newly isolated strains of Streptomycessp.

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An Erratum to this article was published on 01 December 2006

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Abstract

The biosynthesis of poly(ɛ-l-lysine) (ɛ-PL) in the two newly isolated strains of Streptomyceslydicus USE-11 (USE-11) and Streptomyces sp. USE-51 (USE-51) was studied by a newly developed two-stage culture method of cell growth at pH 6.8 and ɛ-PL production at pH 4.5. USE-11 synthesized ɛ-PL consisting of about 28 residues at a high production level, whereas USE-51 did the polymer with 15 ones at a low level. The secreted ɛ-PLs in culture media were digested in a neutral pH range with a peptide hydrolase(s) produced by the ɛ-PL producers. The optimum production levels were presumed to be dependent upon the inherent ɛ-PL synthesis machinery of each producer. The production in USE-51 was sharply dependent upon cell density as was often observed in the production of antibiotics, whereas that in USE-11 was scarcely affected by the density. The \({\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}}\) was found to be essential for the ɛ-PL production in both strains. This might suggest the involvement of a thiol group in the polymerization reactions including the activation of l-lysine. This study indicates that USE-11 is a most suitable strain for the exploration of the ɛ-PL biosynthesis at the molecular level as well as for the technical applications.

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  • 01 December 2006

    The original version of this article unfortunately contained a mistake. The surname of the fourth author was incorrect and should be Ikezaki.

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Acknowledgements

We thank J. Hiraki and M. Hatakeyama of Chisso (Tokyo, Japan) for providing purified ɛ-PL samples, and Y. Hirose of Amano Enzyme (Nagoya, Japan) for the gift of Protease A.

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  1. Department of Materials Science, University of Shiga Prefecture, Hassaka, Hikone, 522-8533, Japan

    Hideo Hirohara, Munenori Takehara, Masayuki Saimura, Atsushi Masayuki & Masahiro Miyamoto

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  1. Hideo Hirohara
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  2. Munenori Takehara
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  4. Atsushi Masayuki
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  5. Masahiro Miyamoto
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Correspondence to Hideo Hirohara.

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Table ESM-1

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Fig. ESM-1

ɛ-PL production (□) and pH value of culturemedium (▣) by fed-batch culture of USE-51 cells growth-cultured for 25 h. Arrows indicate the addition of glycerol to maintain a concentration (■) of 220 mM (GIF 11 kb)

Fig. ESM-2

Effect of concentration of citrate on ɛ-PL production in USE-11 cells growth-cultured for 36 h (GIF 12 kb)

Fig. ESM-3

Proposed pathway of ɛ-PL synthesis in Streptomyces sp. (GIF 11 kb)

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Hirohara, H., Takehara, M., Saimura, M. et al. Biosynthesis of poly(ɛ-l-lysine)s in two newly isolated strains of Streptomycessp. . Appl Microbiol Biotechnol 73, 321–331 (2006). https://doi.org/10.1007/s00253-006-0479-2

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