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Comparative analysis of transcriptional responses to saline stress in the laboratory and brewing strains of Saccharomyces cerevisiae with DNA microarray

  • Genomics and proteomics
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Abstract

To construct yeast strains showing tolerance to high salt concentration stress, we analyzed the transcriptional response to high NaCl concentration stress in the yeast Saccharomycescerevisiae using DNA microarray and compared between two yeast strains, a laboratory strain and a brewing one, which is known as a stress-tolerant strain. Gene expression dynamically changed following the addition of NaCl in both yeast strains, but the degree of change in the gene expression level in the laboratory strain was larger than that in the brewing strain. The response of gene expression to the low NaCl concentration stress was faster than that to the high NaCl concentration stress in both strains. Expressions of the genes encoding enzymes involved in carbohydrate metabolism and energy production in both strains or amino acid metabolism in the brewing strain were increased under high NaCl concentration conditions. Moreover, the genes encoding sodium ion efflux pump and copper metallothionein proteins were more highly expressed in the brewing strain than in the laboratory strain. According to the results of transcriptome analysis, candidate genes for the creation of stress-tolerant strain were selected, and the effect of overexpression of candidate genes on the tolerance to high NaCl concentration stress was evaluated. Overexpression of the GPD1 gene encoding glycerol-3-phosphate dehydrogenase, ENA1 encoding sodium ion efflux protein, and CUP1 encoding copper metallothionein conferred high salt stress tolerance to yeast cells, and our selection of candidate genes for the creation of stress-tolerant yeast strains based on the transcriptome data was validated.

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Acknowledgements

This work was carried out as a project of the Development of a Technological Infrastructure for Industrial Bioprocesses of the New Energy and Industrial Technology Development Organization. This work was supported in part by a grant from the 21st century center of excellence program of the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Osaka, Japan

    T. Hirasawa, Y. Nakakura, K. Ashitani, Y. Katakura & S. Shioya

  2. Institute for Advanced Biosciences, Keio University, 403-1 Nipponkoku, Daihouji, Tsuruoka, 997-0017, Yamagata, Japan

    T. Hirasawa

  3. Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Osaka, Japan

    K. Yoshikawa, K. Nagahisa, C. Furusawa & H. Shimizu

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  1. T. Hirasawa
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  2. Y. Nakakura
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  3. K. Yoshikawa
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  4. K. Ashitani
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  5. K. Nagahisa
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  7. Y. Katakura
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  8. H. Shimizu
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  9. S. Shioya
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Correspondence to S. Shioya.

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Hirasawa, T., Nakakura, Y., Yoshikawa, K. et al. Comparative analysis of transcriptional responses to saline stress in the laboratory and brewing strains of Saccharomyces cerevisiae with DNA microarray. Appl Microbiol Biotechnol 70, 346–357 (2006). https://doi.org/10.1007/s00253-005-0192-6

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  • DOI: https://doi.org/10.1007/s00253-005-0192-6

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