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Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch

  • Applied Genetics and Molecular Biotechnology
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Abstract

Four types of cell-surface-engineered yeast Saccharomyces cerevisiae displaying glucoamylase, namely, systems A, B, C, and D, were constructed to evaluate their performance in direct ethanol fermentation from raw corn starch. Systems A and B were glucoamylase-displaying nonflocculent yeast (YF237) types that secrete α-amylase into the culture medium and codisplay α-amylase on the cell surface, respectively. Systems C and D were flocculent yeast counterparts (YF207) for systems A and B, respectively. In batch fermentations, the specific ethanol production rates of systems A, B, C, and D were 0.18, 0.06, 0.06, and 0.04 g (g cell)−1 h−1, respectively. In repeated fermentations, the specific ethanol production rate of system A decreased with the number of repetitions, whereas, that of system B was maintained. In all systems, the rate-limiting step was the conversion of starch to oligosaccharide because oligosaccharide and glucose were not accumulated throughout the fermentations.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Teik Seong Khaw, Yoshio Katakura & Suteaki Shioya

  2. Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan

    Jun Koh & Akihiko Kondo

  3. Department of Applied Biochemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Mitsuyoshi Ueda

Authors
  1. Teik Seong Khaw
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  2. Yoshio Katakura
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  3. Jun Koh
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  4. Akihiko Kondo
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  5. Mitsuyoshi Ueda
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  6. Suteaki Shioya
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Correspondence to Suteaki Shioya.

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Khaw, T.S., Katakura, Y., Koh, J. et al. Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch. Appl Microbiol Biotechnol 70, 573–579 (2006). https://doi.org/10.1007/s00253-005-0101-z

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  • DOI: https://doi.org/10.1007/s00253-005-0101-z

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