Abstract
The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epithelial cells. Enzyme treatment of bacteria pre-adhered to epithelial cells removed more uropathogenic E. coli than the indigenous flora attached to them.
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References
Choudhury D, Thompson A, Stojanoff V, Langermann S, Pinkner J, Hultgren SJ, Knight SD (1999) X-ray structure of the FimC–FimH chaperone–adhesin complex from uropathogenic Escherichia coli. Science 285:1061–1066
Clegg S (1982) Cloning of genes determining the production of mannose-resistant fimbriae in a uropathogenic strain of Escherichia coli belonging to serogroup O6. Infect Immun 38:739–744
Dodson KW, Pinkner JS, Rose T, Magnusson G, Hultgren SJ, Waksman G (2001) Structural basis of the interaction of the pyelonephritic E. coli adhesin to its human kidney receptor. Cell 105:733–743
Doyle RJ, Oakley JD, Murphy KR, McAlister D, Taylor KG (1985) Graphical analyses of adherence data. In: Mergenhagen SE, Rosan B (eds) Molecular basis of oral microbial adhesion. American Society for Microbiology, Washington, D.C., pp 109–113
Johnson JR (1998) papG alleles among Escherichia coli strains causing urosepsis: associations with other bacterial characteristics and host compromise. Infect Immun 66:4568–4571
Kindinger LE, Koch SR, Doyle RJ, Cowan MM (2001) Asparaginase inhibition of uropathogenic E. coli colonization. Abstr Gen Meet Am Soc Microbiol 101:67–68
Meinders JM, Mei HC van der, Busscher HJ (1995) Deposition efficiency and reversibility of bacterial adhesion under flow. J Colloid Interface Sci 176:329–341
Mobley HLT, Chippendale GR, Warren JW (1995) In vitro adhesion of bacteria to exfoliated uroepithelial cells: criteria for quantitative analysis. Methods Enzymol 253:360–367
Sethman CR, Doyle RJ, Cowan MM (2002) Flow cytometric evaluation of adhesion of Streptococcus pyogenes to epithelial cells. J Microbiol Methods 51:35–42
Stapleton AE, Stroud MR, Hakomori SI, Stamm WE (1998) The globoseries glycosphingolipid sialosyl galactosyl globoside is found in urinary tract tissues and is a preferred binding receptor in vitro for uropathogenic Escherichia coli expressing pap-encoded adhesins. Infect Immun 66:3856–3861
Strömberg N, Marklund B-I, Lund B, Ilver D, Hamers A, Gaastra W, Karlsson KA, Normark S (1990) Host-specificity of uropathogenic Escherichia coli depends on differences in binding specificity to Gal1-4Gal containing isoreceptors. EMBO J 9:2001–2010
Wehner MR, Olchawa MR, Doyle RJ, Cowan MM (2000) The effect of polyphenol oxidase and asparaginase on type 1 and P fimbrial adhesins of uropathogenic Escherichia coli. Abstr Gen Meet Am Soc Microbiol 100:60–61
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This work was supported by Genencor International, Palo Alto, Calif., and the Miami University Office for the Advancement of Scholarship and Teaching.
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Wehner, M.R., Koch, S.R., Kindinger, L. et al. Asparaginase inhibition of adhesion of type 1-fimbriated and P-fimbriated Escherichia coli to epithelial cell receptors. Appl Microbiol Biotechnol 66, 71–73 (2004). https://doi.org/10.1007/s00253-004-1710-7
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DOI: https://doi.org/10.1007/s00253-004-1710-7