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Production of fructosyl transferase by Aspergillus oryzae CFR 202 in solid-state fermentation using agricultural by-products

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Abstract

Fructosyl transferase (FTase) production by Aspergillus oryzae CFR 202 was carried out by solid-state fermentation (SSF), using various agricultural by-products like cereal bran, corn products, sugarcane bagasse,cassava bagasse (tippi) and by-products of coffee and tea processing. The FTase produced was used for the production of fructo-oligosaccharides (FOS), using 60% sucrose as substrate. Among the cereal bran used, rice bran and wheat bran were good substrates for FTase production by A. oryzae CFR 202. Among the various corn products used, corn germ supported maximum FTase production, whereas among the by-products of coffee and tea processing used, spent coffee and spent tea were good substrates, with supplementation of yeast extract and complete synthetic media. FTase had maximum activity at 60°C and pH 6.0. FTase was stable up to 40°C and in the pH range 5.0–7.0. Maximum FOS production was obtained with FTase after 8 h of reaction with 60% sucrose. FTase produced by SSF using wheat bran was purified 107-fold by ammonium sulphate precipitation (30–80%), DEAE cellulose chromatography and Sephadex G-200 chromatography. The molecular mass of the purified FTase was 116.3 kDa by SDS-PAGE. This study indicates the potential for the use of agricultural by-products for the efficient production of FTase enzyme by A. oryzae CFR 202 in SSF, thereby resulting in value addition of those by-products.

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References

  • Acuña-Arguelles M, Gutiérrez-Rojas M, Viniegra-González G, Favela-Torres E (1994) Effect of water activity on exo-pectinase production by A. niger CH4 in solid state fermentation. Biotechnol Lett 16:23–28

    Google Scholar 

  • Alazard D, Raimbault M (1981) Comparative study of amylolytic enzyme production by A. niger in liquid and solid state fermentation. Eur J Appl Microbiol Biotechnol 12:113–117

    CAS  Google Scholar 

  • Alwar ARP, Ramaiah PK (1986) By-products of coffee berries and their possible utilization. Indian Coffee 50:3–8

    Google Scholar 

  • AOAC (1984) Official methods of analysis, 14th edn. Association of Official Analytical Chemists, Washington, D.C.

  • Archana A, Satyanarayana T (1997) Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation. Enzyme Microb Technol 21:12–17

    Article  CAS  Google Scholar 

  • Balasubramaniem AK, Nagarajan KV, Paramasamy G (2001) Process Biochem 36:1241–1247

    Article  CAS  Google Scholar 

  • Battaglino RA, Huergo M, Pilosof AMR, Bartholomai GB (1991) Culture requirements for the production of protease by A. oryzae in solid state fermentation. Appl Microbiol Biotechnol 35:292–296

    CAS  Google Scholar 

  • Boccas F, Roussos S, Gutierrez M, Serrano L, Viniegra G (1994) Production of pectinase from coffee pulp in solid state fermentation system: selection of wild fungal isolate of high potency by a simple three step screening technique. J Food Sci Technol 31:22–26

    CAS  Google Scholar 

  • Deschamps F, Huet MC (1985) Xylanase production in solid state fermentation: a study of its properties. Appl Microbiol Biotechnol 22:177–180

    CAS  Google Scholar 

  • Gabriel O, Wang SF (1969) Determination of enzymatic activity in polyacrylamide gels. I. Enzymes catalyzing the conversion of non-reducing substrates to reducing products. Anal Biochem 27:545–554

    CAS  PubMed  Google Scholar 

  • Hang YD, Woodams EE, Jang KY (1995) Enzymatic conversion of sucrose to kestose by fungal extracellular fructosyl transferase. Biotechnol Lett 17:295–298

    CAS  Google Scholar 

  • Lagemaat JV, Pyle DL (2001) Solid state fermentation and bioremediation: development of a continuous process for the production of fungal tannase. Chem Eng J 84:115–123

    Article  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680–685

    Google Scholar 

  • Martinez Carrera D, Morales P, Sobal M (1988) Cultivation of several Mexican strains of Pleurotus ostreatus on coffee pulp and barley straw. Rev Mex Mycol 4:153–160

    Google Scholar 

  • Pandey A (1992) Recent process developments in solid state fermentation. Process Biochem 27:109–117

    Article  CAS  Google Scholar 

  • Pandey A, Ashakumary L, Selvakumar P, Vijayalakshmi KS (1994). Influence of water activity on growth and activity of A. niger for glucoamylase production in solid-state fermentation. World J Microbiol Biotechnol 10:485–486

    CAS  Google Scholar 

  • Raimbault M, Alazard D (1980) Culture method to study fungal growth in solid state fermentation. Eur J Appl Microbiol Biotechnol 9:199–209

    CAS  Google Scholar 

  • Soares M, Christen P, Pandey A, Soccol CR (2000) Fruity flavor production by Cerratocystis fimbriata grown on coffee husk in solid state fermentation. Process Biochem 35:857–861

    Article  CAS  Google Scholar 

  • Wray W, Boulikas T, Wray VP, Hancock R (1981) Silver staining of proteins in polyacrylamide gels. Anal Biochem 118:197–203

    CAS  PubMed  Google Scholar 

  • Yun (1996) Fructooligosaccharides—occurrence, preparation and application. Enzyme Microb Technol 19:107–117

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful to Dr. Sosaku Ichikawa, Assistant professor, Institute of Applied Biochemistry, University of Tsukuba, Japan, for the generous gift of FOS standards and Mr. Manish Chourasia for the work using corn products. P.T.Sangeetha is thankful to CSIR for the Senior Research Fellowship awarded to her. The authors thank the Director of CFTRI for supporting this work and Mr. A Srinivas, Scientist, Grain Science Technology Department, CFTRI, for the supply of corn products from the Maize Pilot Plant.

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Correspondence to S. G. Prapulla.

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Sangeetha, P.T., Ramesh, M.N. & Prapulla, S.G. Production of fructosyl transferase by Aspergillus oryzae CFR 202 in solid-state fermentation using agricultural by-products. Appl Microbiol Biotechnol 65, 530–537 (2004). https://doi.org/10.1007/s00253-004-1618-2

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  • DOI: https://doi.org/10.1007/s00253-004-1618-2

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