Abstract
The non-conventional yeast Arxula adeninivorans was equipped with the genes phbA, phbB and phbC of the polyhydroxyalkanoate (PHA) biosynthetic pathway of Ralstonia eutropha, which encode β-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively. Arxula strains transformed solely with the PHA synthase gene (phbC) were able to produce PHA. However, the maximum content of the polymer detected in these strains was just 0.003% poly-3-hydroxybutyrate (PHB) and 0.112% poly-3-hydroxyvalerate (PHV). The expression of all three genes (phbA, phbB, phbC) resulted in small increases in the PHA content of the transgenic Arxula cells. However, under controlled cultivation conditions with minimal medium and ethanol as the carbon source, the recombinant yeast was able to accumulate up to 2.2% PHV and 0.019% PHB. Possible reasons for these differences are discussed.
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Acknowledgements
We are grateful to A. Steinbüchel for kindly providing the PHB synthesis genes of R. eutropha. We would also like to thank H. Bohlmann, E. Häusler and I. Schmeling for their excellent technical assistance. This research work was supported by the Deutsche Bundesstiftung Umwelt (AZ 13048) and by funds from Chemical Industry (G.K.).
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Terentiev, Y., Breuer, U., Babel, W. et al. Non-conventional yeasts as producers of polyhydroxyalkanoates—genetic engineering of Arxula adeninivorans . Appl Microbiol Biotechnol 64, 376–381 (2004). https://doi.org/10.1007/s00253-003-1498-x
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DOI: https://doi.org/10.1007/s00253-003-1498-x