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Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application

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Abstract

A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l−1), improved FAEA activity 24.5-fold and a yield of 1 g l−1 of the corresponding protein in the culture medium was achieved. The secreted FAEA was purified 3.5-fold to homogeneity in a two-step purification procedure with a recovery of 69%. The overproduced protein was characterised and presented properties in good agreement with those of native FAEA. The recombinant FAEA was tested for wheat straw pulp bleaching, with or without a laccase mediator system and xylanase. Best results were obtained using a bi-sequential process with a sequence including xylanase, FAEA and laccase, and yielded very efficient delignification—close to 75%—and a kappa number of 3.9. This is the first report on the potential application of recombinant FAEA in the pulp and paper sector.

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Acknowledgements

This work was supported by the Fifth European Framework Program, Quality of Life and Management of Living Resources, key action 1: Food, Nutrition and Health (CROSSENZ : Novel cross-linking enzymes and their consumer acceptance for structure engineering of foods) as well as the GIS-EBL (Conseil Régional Provence-Côte d'Azur and Conseil Général 13, France).

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Authors and Affiliations

  1. UMR 1163 INRA/Université de Provence de Biotechnologie des Champignons Filamenteux, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, 163 avenue de Luminy, Case Postale 925, 13288 , Marseille Cedex 09, France

    E. Record, M. Asther, C. Sigoillot, M. Delattre, M. Haon, J.-C. Sigoillot, L. Lesage-Meessen & Marcel Asther

  2. Laboratoire de Bioénergétique et Ingénierie des Protéines , CNRS UPR9036, 31 chemin Joseph Aiguier, 13402, Marseille Cedex 09, France

    S. Pagès

  3. Université de Provence, 3 place Victor Hugo, 13331, Marseille, France

    S. Pagès

  4. Department of Applied Microbiology and Gene Technology, TNO Nutrition and Food Research Institute, Utrechtseweg 48, P.O. Box 360, 3700 AJ , Zeist, The Netherlands

    P. J. Punt & C. A. M. J. J. van den Hondel

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  1. E. Record
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  2. M. Asther
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  3. C. Sigoillot
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  4. S. Pagès
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  5. P. J. Punt
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  6. M. Delattre
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  7. M. Haon
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  8. C. A. M. J. J. van den Hondel
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  9. J.-C. Sigoillot
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  10. L. Lesage-Meessen
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  11. Marcel Asther
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Correspondence to E. Record.

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Record, E., Asther, M., Sigoillot, C. et al. Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application. Appl Microbiol Biotechnol 62, 349–355 (2003). https://doi.org/10.1007/s00253-003-1325-4

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  • DOI: https://doi.org/10.1007/s00253-003-1325-4

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