Abstract
The combined processes of immunoglobulin (IG) gene rearrangement and somatic hypermutation allow for the creation of an extremely diverse antibody repertoire. Knowledge of the germline sequence of the IG genes is required so that hypermutation and the affinity matured humoral response can be properly studied. Variable region genes can be arranged into subgroups; in humans, there are 11 IGLV subgroups and six IGKV subgroups. The rhesus macaque (Macaca mulatta) is a relevant non-human primate model for human immunological systems. A number of macaque IGHV, IGHD and IGHJ genes have already been reported, but only one light-chain germline gene has been published so far. Here we report the isolation of new macaque IGKV genes by polymerase chain reaction (PCR) amplification from macaque genomic DNA using primers based on the human sequences. Twenty-eight IGKV1, 22 IGKV2 and 12 IGKV3 germline genes for the macaque were found, the open reading frames of which exhibit high homology to their human counterparts (>96, >99 and >96%, respectively).
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Andris JS, Miller AB, Abraham SR, Cunningham S, Roubinet F, Blancher A, Capra JD (1997) Variable region gene segment utilization in rhesus monkey hybridomas producing human red blood cell-specific antibodies: predominance of the VH4 family but not VH4-21 (V4-34). Mol Immunol 34:237–253
Bible JM, Howard W, Robbins H, Dunn-Walters DK (2003) IGHV1, IGHV5 and IGHV7 subgroup genes in the rhesus macaque. Immunogenetics 54:867–873
Bogers WM, Bergmeier LA, Ma J, Oostermeijer H, Wang Y, Kelly CG, ten Haaft P, Singh M, Heeney JL, Lehner T (2004) A novel HIV-CCR5 receptor vaccine strategy in the control of mucosal SIV/HIV infection. AIDS 18:25–36
Bresson D, Chardes T, Chapel N, Bes C, Cerutti M, Devauchelle G, Bouanani M, Mani JC, Peraldi-Roux S (2001) Pertinence of kappa and lambda recombinant antibodies directed against thyroid peroxidase in thyroid autoimmune disease. Hum Antib 10(3–4):109–118
Cannell PK, Amlot P, Attard M, Hoffbrand AV, Foroni L (1994) Variable kappa gene rearrangement in lymphoproliferative disorders: an analysis of V kappa gene usage, VJ joining and somatic mutation. Leukemia 8(7):1139–1145
Chothia C, Gelfand I, Kister A (1998) Structural determinants in the sequences of immunoglobulin variable domain. J Mol Biol 278:457–479
Ermert K, Mitlohner H, Schempp W, Zachau HG (1995) The immunoglobulin kappa locus of primates. Genomics 25:623–629
Giudicelli V, Chaume D, Lefranc M-P (2005) IMGT/GENE-DB: a comprehensive database for human and mouse immunoglobulin and T cell receptor genes. Nucleic Acids Res 33(Database Issue):D256–D261
Gormus BJ, Blanchard JL, Alvarez XH, Didier PJ (2004) Evidence for a rhesus monkey model of asymptomatic tuberculosis. J Med Primatol 33:134–145
Helmuth EF, Letvin NL, Margolin DH (2000) Germline repertoire of the immunoglobulin V(H)3 family in rhesus monkeys. Immunogenetics 51:519–527
Johns MB, Paulus-Thomas JE (1988) Purification of human genomic DNA from whole blood using sodium perchlorate in place of phenol. Anal Biochem 180:276–278
Lefranc M-P (2001) Nomenclature of the human immunoglobulin kappa (IGK) genes. Exp Clin Immunogenet 18:161–174
Lefranc M-P, Lefranc G (2001) The immunoglobulin FactsBook. Academic Press, London
Lefranc M-P, Pommié C, Ruiz M, Giudicelli V, Foulquier E, Truong L, Thouvenin-Contet V, Lefranc G (2003) IMGT unique numbering for immunoglobulin and T cell receptor variable domains and Ig superfamily V-like domains. Dev Comp Immunol 27(1):55–77
Link JM, Hellinger MA, Schroeder HW Jr (2002) The rhesus monkey immunoglobulin IGHD and IGHJ germline repertoire. Immunogenetics 54:240–250
Martinez-Jean C, Folch G, Lefranc M-P (2001) Nomenclature and overview of the mouse (Mus musculus and Mus sp.) immunoglobulin kappa (IGK) genes. Exp Clin Immunogenet 18:255–279
Pommié C, Levadoux S, Sabatier R, Lefranc G, Lefranc M-P (2004) IMGT standardized criteria for statistical analysis of immunoglobulin V-REGION amino acid properties. J Mol Recognit 17:17–32
Prummel MF, Chazenbalk G, Jaume JC, Rapoport B, McLachlan SM (1994) Profile of lambda light chain variable region genes in Graves’ orbital tissue. Mol Immunol 31(11):793–802
Shen Y, Shen L, Sehgal P, Zhou D, Simon M, Miller M, Enimi EA, Henckler B, Chalifoux L, Sehgal N, Gastron M, Letvin NL, Chen ZW (2001) Antiretroviral agents restore Mycobacterium-specific T-cell immune responses and facilitate controlling a fatal tuberculosis-like disease in Macaques coinfected with simian immunodeficiency virus and Mycobacterium bovis BCG. J Virol 75:8690–8696
Shen Y, Zhou D, Chalifoux L, Shen L, Simon M, Zeng X, Lai X, Li Y, Sehgal P, Letvin NL, Chen ZW (2002) Induction of an AIDS virus-related tuberculosis-like disease in macaques: a model of simian immunodeficiency virus- mycobacterium coinfection. Infect Immun 70:869–877
Verthelyi D, Wang VW, Lifson JD, Klinman DM (2004) CpG oligodeoxynucleotides improve the response to hepatitis B immunization in healthy and SIV-infected rhesus macaques. AIDS 18:1003–1008
Wu TT, Kabat EA (1970) An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity. J Exp Med 132:211–250
Acknowledgements
This work was supported by the MRC (J.M.B., E.F.D. and S.O.) and the Guy’s and St Thomas’ Charitable Foundation (W.A.H.). We are grateful for the technical support provided by Ibrahim Dumbuya.
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Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession numbers AY963709-AY963773.
Authors W.A. Howard and J.M. Bible contributed equally to this work.
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Howard, W.A., Bible, J.M., Finlay-Dijsselbloem, E. et al. Immunoglobulin light-chain genes in the rhesus macaque I: kappa light-chain germline sequences for subgroups IGKV1, IGKV and IGKV3. Immunogenetics 57, 210–218 (2005). https://doi.org/10.1007/s00251-005-0794-2
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DOI: https://doi.org/10.1007/s00251-005-0794-2