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Identification of a novel cytokine-like transcript differentially expressed in avian γδ T cells

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Abstract

Chicken represents a species with a high frequency of γδ T cells in peripheral blood, suggesting an important function. To elucidate the genes specific for the avian γδ T cells, the suppression subtractive hybridization (SSH) between γδ and αβ T cells was used. The SSH library, which was successfully enriched for the TCR γ and δ (both V and C region) sequences, provided γδ T-cell-specific genes, including, for example, the ribosomal proteins, signaling and structural molecules, and molecules related to transcription and translation. Among these genes, a clone named KK34 was shown to match the PFAM profile for IL-5 and to have 19.5% amino acid identity to the human interleukin 5 protein. Clone KK34 had lower sequence homology, from 5.4% to 15.6%, to other short-chain four-helix bundle superfamily members IL-3, IL-4, IL-13 and GM-CSF. The hydrophobic signal peptide sequence and the presence of cysteines needed for the interchain disulfide bonds were found to be conserved between clone KK34 and mammalian IL-5 proteins. Clone KK34 transcript expression was studied by RT-PCR, Northern blotting and in situ hybridization and it was confirmed to be expressed in avian γδ T cells. We propose that this clone, KK34, may represent the first nonmammalian IL-5, supporting the findings that γδ T cells are important in the development of allergy.

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Acknowledgements

Ms. A. Karvonen, M. Kuusimurto and J. Matsson are acknowledged for their excellent technical assistance. This project was financially supported by the Academy of Finland, the Finnish Medical Society Duodecim, Turku University Foundation, the Finnish Medical Foundation, Turku Graduate School of Biomedical Sciences and EU program BIOTECH (BIO4-CT97-2706).

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Authors and Affiliations

  1. Department of Medical Microbiology, Turku Graduate School of Biomedical Sciences, University of Turku, Kiinamyllynkatu 13, 20520 , Turku, Finland

    Kimmo Koskela, Pekka Kohonen, Heli Salminen & Olli Lassila

  2. Department of Medical Microbiology, University of Turku, Kiinamyllynkatu 13, 20520 , Turku, Finland

    Tatsuya Uchida

  3. GSF, Institute for Molecular Radiobiology, Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany

    Jean-Marie Buerstedde

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  1. Kimmo Koskela
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Correspondence to Kimmo Koskela.

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Koskela, K., Kohonen, P., Salminen, H. et al. Identification of a novel cytokine-like transcript differentially expressed in avian γδ T cells. Immunogenetics 55, 845–854 (2004). https://doi.org/10.1007/s00251-004-0643-8

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  • DOI: https://doi.org/10.1007/s00251-004-0643-8

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