Abstract
Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the electrophoretic mobility (EPM) of single, optically trapped yeast cells under hyperosmotic stress conditions using optical tweezers combined with a position detector. We studied the dynamics of the EPM stress response for cells at different phases of the cell cycle.
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Acknowledgments
We thank M. Tonin for fruitful discussions, S. Balint for his help in preparation of cells and medium, V. Petrov for his help in manuscript preparation, and Fundació Privada Cellex Barcelona, the Spanish Ministry of Science and Innovation (MICINN FIS2008-00114), and Catalunya Caixa for financial support.
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Mestres, P., Petrov, D. Real-time detection of changes in the electrophoretic mobility of a single cell induced by hyperosmotic stress. Eur Biophys J 40, 1081–1085 (2011). https://doi.org/10.1007/s00249-011-0725-0
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DOI: https://doi.org/10.1007/s00249-011-0725-0