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Attachment of β2-glycoprotein I to negatively charged liposomes may prevent the release of daughter vesicles from the parent membrane

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Abstract

The temperature-induced budding of POPC–cardiolipin–cholesterol, POPC–POPS–cholesterol and POPC–POPG–cholesterol giant lipid vesicles in the presence of β2-glycoprotein I (β2-GPI) in the outer solution was studied experimentally and theoretically. The observed budding transition of vesicles was continuous which can be explained by taking into account the orientational ordering and direct interactions between oriented lipids. The attachment of positively charged β2-GPI to the negatively charged outer surface of POPC–cardiolipin–cholesterol, POPC–POPS–cholesterol and POPC–POPG–cholesterol giant vesicles caused coalescence of the spheroidal membrane bud with the parent vesicle before the bud could detach from the parent vesicle, i.e. vesiculate. Theoretically, the protein-mediated attraction between the membrane of a bud and the parent membrane was described as an interaction between two electric double layers. It was shown that the specific spatial distribution of charge within β2-GPI molecules attached to the negatively charged membrane surface may explain the observed attraction between like-charged membrane surfaces.

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Acknowledgments

This work was supported by ARRS grants J3-9219-0381-06 and P2-0232-1538.

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Authors and Affiliations

  1. Laboratory of Clinical Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia

    Jasna Urbanija, Nejc Tomšič & Veronika Kralj-Iglič

  2. Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

    Blaž Babnik, Veronika Kralj-Iglič & Aleš Iglič

  3. Department of Rheumatology, University Medical Centre Ljubljana, Ljubljana, Slovenia

    Mojca Frank & Blaž Rozman

  4. Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000, Ljubljana, Slovenia

    Aleš Iglič

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  1. Jasna Urbanija
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  2. Blaž Babnik
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  3. Mojca Frank
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  4. Nejc Tomšič
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  6. Veronika Kralj-Iglič
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Correspondence to Aleš Iglič.

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Regional Biophysics Conference of the National Biophysical Societies of Austria, Croatia, Hungary, Italy, Serbia, and Slovenia.

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Urbanija, J., Babnik, B., Frank, M. et al. Attachment of β2-glycoprotein I to negatively charged liposomes may prevent the release of daughter vesicles from the parent membrane. Eur Biophys J 37, 1085–1095 (2008). https://doi.org/10.1007/s00249-007-0252-1

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  • DOI: https://doi.org/10.1007/s00249-007-0252-1

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