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Soil Microbial Community Responses After Amendment with Thermally Altered Pinus radiata Needles

  • Soil Microbiology
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Abstract

Post-fire litter layers are composed of leaves and woody debris that predominantly fall during or soon after the fire event. These layers are distinctly different to pre-fire litters due to their common origin and deposition time. However, heterogeneity can arise from the variable thermal conditions in the canopy during fire. Therefore, in this study, we used thermally altered pine needles (heated to 40 °C, 150 °C, 260 °C and 320 °C for 1 h) in a laboratory incubation study for 43 days. These samples were measured for respiration throughout and extracted for DNA at the experiment’s end; soil ribosomal RNA was analysed using Illumina sequencing (16S and internal transcribed spacer amplicons). The addition of pine needles heated to 40 °C or 150 °C caused a substantial shift in community structure, decreased alpha diversity and significantly increased soil respiration relative to the control treatment. In contrast, pine needles heated to 260 °C or 320 °C had little effect on microbial community structure or soil respiration. These results indicate that highly thermally altered needles are not microbially decomposed during the first 43 days of exposure and therefore that biomass temperature may have significant effects on post-fire litter decomposition and carbon flux. This research outlines an important knowledge gap in forest fire responses that may affect post-fire carbon emission estimates.

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Acknowledgements

This project was supported by the Holsworth Wildlife Research Endowment and the Ecological Society of Australia, and the University of Adelaide Research Training Program Scholarship, with further analytical assistance provided by the CSIRO Agriculture and Food. We thank the editor and reviewers for their constructive comments on our original submission.

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Authors and Affiliations

  1. School of Agriculture, Food and Wine, and The Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064, Australia

    Erinne Stirling, Lynne M. Macdonald, Ronald J. Smernik & Timothy R. Cavagnaro

  2. CSIRO Agriculture and Food, Waite Campus, Glen Osmond, South Australia, 5064, Australia

    Erinne Stirling, Lynne M. Macdonald, Ronald J. Smernik & Timothy R. Cavagnaro

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Solid-state 13C CP-MAS NMR spectra for pine needles heated to 4 temperatures. Values are single measurements. Vertical grey lines indicate boundaries of chemical shift regions: 0-45 ppm (alkyl C), 45-60 ppm (N-alkyl C), 60-110 ppm (O-alkyl C), 110-145 ppm (aryl C), 145-165 ppm (O-aryl-C), and 165-215 ppm (carbonyl C). (DOCX 55 kb)

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Stirling, E., Macdonald, L.M., Smernik, R.J. et al. Soil Microbial Community Responses After Amendment with Thermally Altered Pinus radiata Needles. Microb Ecol 79, 409–419 (2020). https://doi.org/10.1007/s00248-019-01402-x

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