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Differentiated Response of Denitrifying Communities to Fertilization Regime in Paddy Soil

  • Soil Microbiology
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Abstract

The impact of fertilization regimes on sequential denitrifying communities was investigated in a rice paddy field with 17 years continuous fertilization, located in Taoyuan Agro-ecosystem Research Station (110°72″ E, 28°52″ N), China. The diversity, community composition, and size of denitrifying genes of narG, qnorB, and nosZ were determined using molecular tools including terminal restriction fragment length polymorphism, quantitative polymerase chain reaction (qPCR), cloning, and sequencing analysis. Soil samples were collected from the plots with no fertilizer (NF), urea (UR), balanced mineral fertilizers (BM), and BM combined with rice straw (BMR). UR and BM caused marked increase in the community size of the denitrifying genes; however, BMR resulted in the highest abundance. The community size of narG was the most affected by the fertilization regimes, while qnorB was the least. Fertilization also induced some shifts in the composition of denitrifying genes, but the responses of different genes varied. However, fertilization regimes caused no significant changes to the diversity of the denitrifying genes. Potential denitrification activity (PDA) was significantly correlated with the abundance of narG and nosZ rather than qnorB, but there were no such correlations between PDA and the composition and diversity of denitrifying communities. Conclusively, long-term fertilization significantly affected denitrifying community size and composition, but not diversity. Among the sequential denitrifying genes, narG was the most, while qnorB was the least sensitive communities to fertilization regimes.

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Acknowledgments

We thank Professor Min Lin for providing Pseudomonas stutzeri A1501 and Professor Ron McLaren for English improvements. This work was financially supported by KZCX2-YW-BR-01, KZCX2-YW-T07, NSFC 41090282 and 41071181.

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Author notes

  1. Minna Wu

    Present address: Xinxiang Medical University, Xinxiang, 450003, China

Authors and Affiliations

  1. Key Laboratory of Agro-ecological Processes in Subtropical Regions and Taoyuan Agro-ecosystem Research Station, Soil Molecular Ecology Section, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China

    Zhe Chen, Jinbo Liu, Minna Wu, Xiaoli Xie, Jinshui Wu & Wenxue Wei

  2. State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry (LAPC), Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing, 100029, China

    Zhe Chen

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Jinbo Liu

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  1. Zhe Chen
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Correspondence to Wenxue Wei.

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Chen, Z., Liu, J., Wu, M. et al. Differentiated Response of Denitrifying Communities to Fertilization Regime in Paddy Soil. Microb Ecol 63, 446–459 (2012). https://doi.org/10.1007/s00248-011-9909-5

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