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How a Bacterial Community Originating from a Contaminated Coastal Sediment Responds to an Oil Input

  • Environmental Microbiology
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Abstract

Bacterial communities inhabiting coastal sediments are subjected to oil spills. In order to examine the early structural response of a complex bacterial community to oil pollution, a kinetic study of the crude oil impact on bacterial communities inhabiting sediments from the contaminated Etang-de-Berre lagoon was performed. The sediments were maintained in slurries in presence or absence of crude oil and the kinetic study was carried out 14 days. During this period, 54% of crude oil was biodegraded showing the importance of the early degradation step. The metabolically active community (16S rRNA transcript analysis) was immediately impacted by the oil input, observed as an apparent decrease of species richness in the first hour of incubation. Nevertheless, this shift was quickly reversed, highlighting a fast, adaptative and efficient response of the metabolically active bacterial population. The high proportion of sequences related to hydrocarbonoclastic strains or petroleum-associated clones in active oiled community was consistent with significant increasing numbers of cultivable hydrocarbonoclastic bacteria at the end of the experiment. We concluded that “Etang-de-Berre” bacterial communities inhabiting oiled sediments for decades adopted a specific structure depending on oil presence and were able to face hydrocarbon contamination quickly and efficiently.

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Acknowledgments

This work was funded by the European Community Project FACEiT [STREP- grant N° 018391 (GOCE)]. We would like to thank all partners of the FACEiT project for their useful discussions. We acknowledge the financial support by the Aquitaine Regional Government Council (France), the Ministère de l’Ecologie et du Développement Durable (MEDD - PNETOX project N° CV04000147) and the ANR (DHYVA project, No. 06SEST09). The authors are especially indebted to Dr Anne Fahy for valuable comments on the manuscript and careful reading of the English.

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Authors and Affiliations

  1. Equipe Environnement et Microbiologie-UMR CNRS IPREM 5254, Université de Pau, BP1155, 64013, Pau Cedex, France

    Sandrine Païssé, Marisol Goñi-Urriza & Robert Duran

  2. Centre for Resource Management and Efficiency, School of Applied Sciences, Department of Sustainable Systems, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK

    Frédéric Coulon

Authors
  1. Sandrine Païssé
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  2. Marisol Goñi-Urriza
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  3. Frédéric Coulon
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  4. Robert Duran
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Corresponding author

Correspondence to Robert Duran.

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Table S1

Changes in oil hydrocarbons fraction abundance and concentrations (as % of initial values) over 14 days. Each percentage represents the mean of hydrocarbon fractions from replicate samples. (DOC 77 kb)

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Païssé, S., Goñi-Urriza, M., Coulon, F. et al. How a Bacterial Community Originating from a Contaminated Coastal Sediment Responds to an Oil Input. Microb Ecol 60, 394–405 (2010). https://doi.org/10.1007/s00248-010-9721-7

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  • DOI: https://doi.org/10.1007/s00248-010-9721-7

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