Abstract.
Citrate transport into the vacuoles of acid lime juice cells was investigated using isolated tonoplast vesicles. ATP stimulated citrate uptake in the presence or in the absence of a ΔμH+. Energization of the vesicles only by an artificial K+ gradient (establishing an inside-positive Δψ) also resulted in citrate uptake as was the case of a ΔpH dominated ΔμH+. Addition of inhibitors to endomembrane ATPases showed no direct correlation between the inhibition to the tonoplast bound H+/ATPase and citrate uptake. The data indicated that, although some citrate uptake can be accounted for by Δψ and by a direct primary active transport mechanism involving ATP, under in vivo conditions of vacuolar pH of 2.0, citrate uptake is driven by ΔpH.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 27 April 1998/Revised: 8 September 1998
Rights and permissions
About this article
Cite this article
Brune, A., Gonzalez, P., Goren, R. et al. Citrate Uptake into Tonoplast Vesicles from Acid Lime (Citrus aurantifolia) Juice Cells. J. Membrane Biol. 166, 197–203 (1998). https://doi.org/10.1007/s002329900461
Issue Date:
DOI: https://doi.org/10.1007/s002329900461