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Supported Planar Single and Multiple Bilayer Formation by DOPC Vesicle Rupture on Mica Substrate: A Mechanism as Revealed by Atomic Force Microscopy Study

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Abstract

A planar lipid bilayer on a solid support serves as model system that explains fundamental aspects of membrane biology and enables us to characterize wide-range surface-sensitive techniques, including molecular engineering. The present study aims at understanding the process of single and multiple bilayer formation after the exposure of small unilamellar vesicles (SUVs) of dioleoyl phosphatidylcholine (DOPC) to mica substrate. Isolated single bilayer formation and co-existence of double and triple lipid bilayers in the aqueous medium have been quantitatively measured by atomic force microscopy and discussed the physicochemical mechanism. It has been observed that due to the strong adhesion of DOPC SUV to mica surface, vesicles of diluted solution rupture spontaneously and form isolated bilayer patches when they come in contact with the mica surface. No further lateral growth or movement of the bilayer patches has been observed upon increase of incubation time. However, the increase of vesicle number on the same surface area by successive deposition of DOPC solution of same concentration and increasing incubation time shows merging of the nearby patches as well as development of stacked second and third bilayers due to edge-guided rupture of adsorbed vesicles on first or second bilayer patches. Mechanisms of single and multi-bilayer formation and a theoretical interpretation of the process have been elucidated.

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Acknowledgements

This work has been financially supported DBT funded Research Project (BT/PR8475/BRB/10/1248/2013). The authors are grateful to VECC Kolkata, DAE, Govt. of India for providing AFM Facility to carry out the research. The authors would like to acknowledge Pabitra Maity and Animesh Halder for their help during vesicle preparation and DLS measurements. They specially thank Ms. Sanhita Mukherjee, Arijit Chakrabarty, and Pratibho Karmakar for critical reading of this manuscript.

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  1. Soft Matter and Biophysics Laboratory, Department of Physics, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, 700032, India

    Amrita Basu & Sanat Karmakar

  2. RIB Group, Variable Energy Cyclotron Centre, 1/AF, Bidhannagar, Kolkata, 700064, India

    Prasanta Karmakar

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  1. Amrita Basu
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Correspondence to Prasanta Karmakar.

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Basu, A., Karmakar, P. & Karmakar, S. Supported Planar Single and Multiple Bilayer Formation by DOPC Vesicle Rupture on Mica Substrate: A Mechanism as Revealed by Atomic Force Microscopy Study. J Membrane Biol 253, 205–219 (2020). https://doi.org/10.1007/s00232-020-00117-2

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