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Enterodiol is Actively Transported by Rat Liver Cell Membranes

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Abstract

The interaction of enterodiol and the well-described polyphenol epigallocatechin gallate (EGCG) with hepatic membranes has been matter of interest in the last few years. On one hand, EGCG is only able to bind to the phospholipid polar head groups, as it has been already described in synthetic lipid bilayers and erythrocyte membranes but cannot get inserted into the hydrophobic core or be transported into the lumen of membrane vesicles. On the other, enterodiol has no interaction with non-energized membranes either, but it is able to interact and even be transported upon addition of ATP. In fact, the ATPase activity undergoes a twofold increase in the presence of enterodiol but not in the presence of EGCG. This is the first report on the transport of enterodiol by liver membranes, and it may help explain the rather high blood concentrations of this estrogenic enterolignan compared to EGCG, which is extensively metabolized by the intestine and the liver. The present results suggest that a fraction of enterodiol may escape the liver inactivation by being pumped out from the hepatocytes to the bloodstream.

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Acknowledgements

This work was supported by CONICET (Grant PIP 0183), Agencia (Grant PICT 2012 N°2998), and UNT (Grant PIUNT 2014 D548/1). A.A.M.C. and P.B.S. are recipients of a CONICET fellowship. C.M. is a career investigator of CONICET. The authors would like to thank N.S. Rios Colombo for constructive criticism of the manuscript.

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  1. Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICETUNT, and Instituto de Química Biológica “Dr. Bernabé Bloj”, Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI, San Miguel de Tucumán, Argentina

    Alejandro de Athayde Moncorvo Collado, Paula B. Salazar & Carlos Minahk

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  1. Alejandro de Athayde Moncorvo Collado
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  2. Paula B. Salazar
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  3. Carlos Minahk
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Correspondence to Carlos Minahk.

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de Athayde Moncorvo Collado, A., Salazar, P.B. & Minahk, C. Enterodiol is Actively Transported by Rat Liver Cell Membranes. J Membrane Biol 251, 593–600 (2018). https://doi.org/10.1007/s00232-018-0035-x

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