Abstract
Objective
The findings of numerous studies have suggested that both genetic and environmental influences are involved in the pathogenesis of allergic disease and atopy. We studied the polymorphisms in the interferon (IFN)-gamma (γ) and IFN-γ receptor 1 (IFNR1) gene with the aim of clarifying the relationships among these polymorphisms, penicillin allergy and anti-penicillin antibodies.
Methods
A restriction endonuclease fragment length polymorphism (RFLP)-PCR analysis and sequencing were used to study the IFNR1 and IFN-γ polymorphisms. The presence and level of eight specific immunoglobulin (Ig)E and IgG antibodies were determined by the radioallergosorbent test (RAST) and enzyme-linked immunosorbent assay (ELISA), respectively.
Results
The positive rates of specific IgE and IgG were 61.11 and 53.92%, respectively. There was no significant difference in the whole-allele of IFN-γ distribution between patients with a penicillin allergy and control subjects. Allele 7 (18CA repeat) was significantly less frequent in the urticaria group (3.19 vs. 11.93%) than in the controls. There was no difference in IFN-γ production among different alleles in IFN-γ. The frequency of G/A (Val/Met) in the IFNR1 gene in allergic patients was significantly less than that in the controls (P < 0.05). There were no significant differences in the positive rate of IgE among different alleles of IFN-γ. The same was true for the positive rate of IgG.
Conclusions
The Met/Val allele in IFNR1 gene may have a protective role in the non-penicillin allergic population. The allele 18CA repeat in IFN-γ gene may be associated with urticaria.
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Acknowledgements
We thank Professor BA Baldo and Doctor ZJ Zhao (Molecular Immunology Unit, Kolling Institute of Medical Research, Royal North Shore Hospital of Sydney, Australia) for their excellent technical assistance. This project was supported by the Science Foundation for Distinguished Young Scholars of Henan Province (No. 0312002100) and the Engineering Project for Innovative Scholars of Henan Province (No. 2002114).
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Gao, N., Qiao, HL., Jia, LJ. et al. Relationships between specific serum IgE, IgG, IFN-γ level and IFN-γ, IFNR1 polymorphisms in patients with penicillin allergy. Eur J Clin Pharmacol 64, 971–977 (2008). https://doi.org/10.1007/s00228-008-0486-6
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DOI: https://doi.org/10.1007/s00228-008-0486-6