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Supplementation with Green Tea Polyphenols Improves Bone Microstructure and Quality in Aged, Orchidectomized Rats

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Abstract

Recent studies show that green tea polyphenols (GTPs) attenuate bone loss and microstructure deterioration in ovariectomized aged female rats, a model of postmenopausal osteoporosis. This study evaluated the efficacy of GTPs at mitigating bone loss and microstructure deterioration along with related mechanisms in androgen-deficient aged rats, a model of male osteoporosis. A 2 (sham vs. orchidectomy) × 2 (no GTP and 0.5% GTP in drinking water) factorial design was studied for 16 weeks using 40 aged male rats. An additional 10 rats (baseline group) were killed at the beginning of study to provide baseline parameters. There was no difference in femoral mineral density between baseline and the sham only group. Orchidectomy suppressed serum testosterone and tartrate-resistant acid phosphatase concentrations, liver glutathione peroxidase activity, bone mineral density, and bone strength. Orchidectomy also decreased trabecular bone volume, number, and thickness in the distal femur and proximal tibia and bone-formation rate in trabecular bone of proximal tibia but increased serum osteocalcin concentrations and bone-formation rates in the endocortical tibial shaft. GTP supplementation resulted in increased serum osteocalcin concentrations, bone mineral density, and trabecular volume, number, and strength of femur; increased trabecular volume and thickness and bone formation in both the proximal tibia and periosteal tibial shaft; decreased eroded surface in the proximal tibia and endocortical tibial shaft; and increased liver glutathione peroxidase activity. We conclude that GTP supplementation attenuates trabecular and cortical bone loss through increasing bone formation while suppressing bone resorption due to its antioxidant capacity.

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Acknowledgement

This study was supported by Winthrop-University Hospital and Texas Tech University Health Sciences Centers.

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Authors and Affiliations

  1. Department of Pathology, Texas Tech University Health Sciences Center, BB 198, 3601 4th street, Lubbock, TX, 79430, USA

    Chwan-Li Shen, Raul Y. Dagda & Ming-Chien Chyu

  2. Laura W. Bush Institute for Women’s Health, Texas Tech University, Lubbock, TX, 79430, USA

    Chwan-Li Shen

  3. USDA ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, 58201, USA

    Jay J. Cao

  4. Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX, 79430, USA

    Thomas E. Tenner Jr.

  5. Department of Mechanical Engineering, Texas Tech University, Lubbock, TX, 79401, USA

    Ming-Chien Chyu

  6. Graduate Healthcare Engineering Option, Texas Tech University, Lubbock, TX, 79401, USA

    Ming-Chien Chyu

  7. Applied Bench Core Laboratory, Winthrop-University Hospital, Mineola, NY, 11501, USA

    James K. Yeh

Authors
  1. Chwan-Li Shen
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  2. Jay J. Cao
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  3. Raul Y. Dagda
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  4. Thomas E. Tenner Jr.
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  5. Ming-Chien Chyu
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  6. James K. Yeh
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Corresponding author

Correspondence to Chwan-Li Shen.

Additional information

Partial results were communicated at the Annual Meeting of American Society for Bone and Mineral Research.

The authors have stated that they have no conflict of interest.

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Shen, CL., Cao, J.J., Dagda, R.Y. et al. Supplementation with Green Tea Polyphenols Improves Bone Microstructure and Quality in Aged, Orchidectomized Rats. Calcif Tissue Int 88, 455–463 (2011). https://doi.org/10.1007/s00223-011-9477-1

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  • DOI: https://doi.org/10.1007/s00223-011-9477-1

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