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Induced Apoptosis of Chondrocytes by Porphyromonas gingivalis as a Possible Pathway for Cartilage Loss in Rheumatoid Arthritis

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Abstract

The role of bacterial infections in the pathogenesis of rheumatoid arthritis (RA) has gained increasing interest. Patients with RA often exhibit periodontal disease, which is associated with pathogens like Porphyromonas gingivalis. The present study examines the direct effects of P. gingivalis on apoptosis of human chondrocytes (a feature of inflammatory joint diseases) as one can assume an interrelation of pathogenesis of RA and P. gingivalis infections. Primary chondrocytes were infected with P. gingivalis. Early apoptotic and dead cell analysis was performed using Annexin-V, 7AAD, and propidium iodide and examined by flow cytometry and fluorescence microscopy. Caspase activation and DNA fragmentation were determined by western blot analysis and TUNEL reaction. Flow cytometry and fluorescence microscopy demonstrated an increase of Annexin-V-positive early apoptotic chondrocytes after infection. Western blot showed upregulation of activated caspase-3 expression, and TUNEL reaction revealed considerable DNA fragmentation following infection. The data show that P. gingivalis promotes early and later stages of apoptosis of primary human chondrocytes, which might contribute to the joint damage seen in the pathogenesis of RA.

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Acknowledgements

This work was financially supported DFG GK 325 (Bonn, Germany), by DGP e.V. (Germany), GABA GmbH (Lörrach, Germany), and a Habilitation Fellowship from Charité-Universitätsmedizin (Berlin, Germany to N. P.).

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Authors and Affiliations

  1. Department of Rheumatology and Clinical Immunology, Charité University Hospital, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany

    E. Röhner, J. Detert, P. Kolar, G. R. Burmester & F. Buttgereit

  2. Department of Infectious Diseases and Pneumology, Charité University Hospital, Berlin, Germany

    A. Hocke & P. N’Guessan

  3. Department of Orthopaedics, Charité University Hospital, Berlin, Germany

    G. Matziolis

  4. Department of Operative Dentistry and Periodontology, Charité University Hospital, Berlin, Germany

    V. Kanitz, J. P. Bernimoulin, A. Kielbassa & N. Pischon

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  1. E. Röhner
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  2. J. Detert
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  3. P. Kolar
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  4. A. Hocke
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  5. P. N’Guessan
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  6. G. Matziolis
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  7. V. Kanitz
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  8. J. P. Bernimoulin
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  9. A. Kielbassa
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  10. G. R. Burmester
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  11. F. Buttgereit
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  12. N. Pischon
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Correspondence to E. Röhner.

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E. Röhner and J. Detert contributed equally to this work.

The authors have stated that they have no conflict of interest.

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Röhner, E., Detert, J., Kolar, P. et al. Induced Apoptosis of Chondrocytes by Porphyromonas gingivalis as a Possible Pathway for Cartilage Loss in Rheumatoid Arthritis. Calcif Tissue Int 87, 333–340 (2010). https://doi.org/10.1007/s00223-010-9389-5

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  • DOI: https://doi.org/10.1007/s00223-010-9389-5

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