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The Nitric Oxide Donor Sodium Nitroprusside Inhibits Mineralization in ATDC5 Cells

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Abstract

The aim of this study was to test whether the nitric oxide (NO) donor sodium nitroprusside (SNP) has an effect on mineralization in ATDC5 cells. Mineralization in ATDC5 cell culture was induced by addition of β-glycerophosphate or inorganic phosphate, visualized by staining precipitated calcium with an alizarin red stain, and quantified using atomic absorption spectrometry. SNP was shown to inhibit the mineralization of ADTC5 cells. This inhibition was not affected by inhibitors of guanylyl cyclase nor mimicked by a cyclic guanosine monophosphate (cGMP) analog. Furthermore, SNP did not inhibit phosphate uptake or inhibit apoptosis in ATDC5 cells. These findings indicate that SNP can specifically inhibit matrix mineralization via a cGMP-independent pathway and that the effect is not mediated by inhibition of phosphate transport or apoptosis. These results suggest a preventive role of NO in premature or pathological mineralization.

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Acknowledgments

We thank Hugo S. Wouterse for expert assistance with the atomic absorption spectroscopy. We also thank the Centre for Cell Imaging at the Faculty of Veterinary Medicine, Utrecht University, for use of the fluorescence microscope.

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Authors and Affiliations

  1. Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Graduate School of Animal Health, Utrecht University, P.O. Box 80.176, Utrecht, TD, NL-3508, The Netherlands

    L. F. A. Huitema, A. B. Vaandrager, J. B. Helms & C. H. A. van de Lest

  2. Department of Equine Sciences, Faculty of Veterinary Medicine, Graduate School of Animal Health, Utrecht University, P.O. Box 80.153, Utrecht, TD, NL-3508, The Netherlands

    L. F. A. Huitema, P. R. van Weeren, A. Barneveld & C. H. A. van de Lest

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  1. L. F. A. Huitema
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Correspondence to C. H. A. van de Lest.

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Huitema, L.F.A., Vaandrager, A.B., van Weeren, P.R. et al. The Nitric Oxide Donor Sodium Nitroprusside Inhibits Mineralization in ATDC5 Cells. Calcif Tissue Int 78, 171–177 (2006). https://doi.org/10.1007/s00223-005-1233-y

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  • DOI: https://doi.org/10.1007/s00223-005-1233-y

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