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Ultrastructure of Dental Enamel afflicted with Hypoplasia: An Atomic Force Microscopic Study

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Abstract

The ultrastructure of the human tooth enamel from a patient diagnosed with hypoplasia (HYP) was investigated using atomic force microscopy (AFM) and compared with the surface of normal human tooth enamel. Hypoplasia is a hereditary defect of dental enamel in which the enamel is deficient in either quality or quantity. AFM results presented for the HYP tooth enamel clearly demonstrate that the apatite crystal morphology in hypoplasia tooth enamel is perturbed in the diseased state which could result from a defective synthesis of the extracellular matrix proteins, e.g., amelogenin, by the ameloblasts. HYP enamel consisting of loosely packed, very small grains does not present a tendency for association, as in the case of the normal healthy tooth. Indeed, the enamel surface affected by HYP is porous and is made of much smaller grains. In some samples, the HYP part of enamel surface appeared in the form of a point-defect, which we believe may be associated with the early stages of the HYP deformation.

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Acknowledgements

This research was partially supported by a grant from the National Institutes of Health (V.R.). V.R. acknowledges partial financial support from CONACyT during 1998–99. N.B. was supported by CONACyT grants, 0913E-P and L0081-E9608.

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Authors and Affiliations

  1. Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, 09340 México DF, Mexico

    N. Batina & M. Morales

  2. Children’s Hospital, Harvard Medical School, Boston, MA 02115, USA

    V. Renugopalakrishnan

  3. Department of Biomedical Engineering, Florida International University, Miami, FL 33174, USA

    V. Renugopalakrishnan & S. L. Lakka

  4. Centre de Ciencias Físicas, Universidad Nacional Autónoma de México, 62210 Cuernavaca, Morelos, Mexico

    P. N. Casillas Lavín, J. C. H. Guerrero & R. Garduño-Juárez

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  1. N. Batina
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  2. V. Renugopalakrishnan
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  3. P. N. Casillas Lavín
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  4. J. C. H. Guerrero
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  5. M. Morales
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  6. R. Garduño-Juárez
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  7. S. L. Lakka
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Correspondence to V. Renugopalakrishnan.

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Batina, N., Renugopalakrishnan, V., Casillas Lavín, P. et al. Ultrastructure of Dental Enamel afflicted with Hypoplasia: An Atomic Force Microscopic Study . Calcif Tissue Int 74, 294–301 (2004). https://doi.org/10.1007/s00223-002-1045-2

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  • DOI: https://doi.org/10.1007/s00223-002-1045-2

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