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bFGF Administration Lowers the Phosphate Threshold for Mineralization in Bone Marrow Stromal Cells

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Abstract

Basic fibroblast growth factor (bFGF) is a potent mitogen and acts as an autocrine/paracrine factor for osteoblasts. Long-term administration of bFGF in vivo increases osteoblast number and stimulates matrix formation, but induces hypophosphatemia and impairs matrix mineralization. The goal of this study was to examine the interaction between bFGF and low levels of organic phosphate in an effort to better understand the possible long-term therapeutic effects of bFGF. These data show that in vitro administration of bFGF accelerates the calcification process and lowers the phosphate threshold needed for successful bone nodule formation. This correlates well with the observed upregulation of mRNA production for alkaline phosphatase and osteocalcin at day 7. These findings help elucidate the mechanisms of bFGF action on bone marrow stromal cell differentiation and mineralization and indicate that the delay in mineralization observed in vivo may not be caused by decreased phosphate availability alone.

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Acknowledgements

Support for this work was supplied by the National Science Foundation (NSF BES-9820113), NASA NAG2-1371, and the Miller Institute for Basic Research in Science, Berkeley, CA. Additional support was provided by the National Defense Science and Engineering Graduate Fellowship Program and the Berkeley Fellowship Program (E.A.N.).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Tulane University, New Orleans, LA 70118, USA

    E. A. Nauman

  2. Veterans Affairs Medical Center, San Francisco, CA 94121, USA

    E. A. Nauman, T. Sakata, B. P. Halloran & D. D. Bikle

  3. Department of Medicine, University of California, San Francisco, CA 94143, USA

    T. Sakata, B. P. Halloran & D. D. Bikle

  4. Orthopaedic Biomechanics Laboratory, Department of Mechanical Engineering, University of California, Berkeley, CA 94720-1740, USA

    E. A. Nauman & T. M. Keaveny

  5. Department of Orthopaedic Surgery, University of California, San Francisco, CA 94143, USA

    T. M. Keaveny

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  1. E. A. Nauman
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  2. T. Sakata
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  3. T. M. Keaveny
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  4. B. P. Halloran
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  5. D. D. Bikle
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Correspondence to E. A. Nauman.

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Nauman, E., Sakata, T., Keaveny, T. et al. bFGF Administration Lowers the Phosphate Threshold for Mineralization in Bone Marrow Stromal Cells . Calcif Tissue Int 73, 147–152 (2003). https://doi.org/10.1007/s00223-002-1033-6

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  • DOI: https://doi.org/10.1007/s00223-002-1033-6

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