Abstract
Peripheral inflammation and nerve injury usually accompany each other. However, whether inflammatory and neuropathic pain share similar mechanisms at all stages is unknown. TRPV1 and P2X3 are two major ion channels in dorsal root ganglia (DRGs) and are involved in chronic pain. Here, their function and expression in DRGs at different phases of the two types of pain were investigated. Both the paw withdrawal threshold (PWT) and paw withdrawal latency were decreased in rats injected with complete Freud’s adjuvant (CFA). However, only the PWT was decreased in rats with spared nerve injury (SNI). CFA increased the magnitude of the TRPV1-mediated Ca2+ response but not the P2X3-mediated Ca2+ response 14 days after injection. Consistent with this result, the P2X3 expression level in CFA rats was increased only at 3 days after injection. SNI surgery increased the magnitudes of the TRPV1- and P2X3-mediated Ca2+ responses and upregulated both TRPV1 and P2X3 expression in lumbar DRGs. The distributions of TRPV1 and P2X3 in DRGs after modeling were observed, and TRPV1 was found to be highly expressed mainly in the L4–L5 DRGs in CFA rats and in the L5–L6 DRGs in SNI rats. P2X3 was highly expressed in the L4–L6 DRGs in CFA rats 3 days after injection but was only highly expressed in the L4 DRG 14 days after modeling. On the other hand, SNI promoted the P2X3 expression L4–L5 DRGs 3 days after surgery, but only L6 DRG 14 days after modeling. All the results indicate that P2X3 and TPRV1 are involved in inflammatory and neuropathic pain by different expression levels and distributions in the lumbar DRG in the chronic stage.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- P2X3:
-
P2X purinoceptor 3
- DRG:
-
Dorsal root ganglion
- TRPV1:
-
Transient receptor potential cation channel subfamily V member 1
- CAP:
-
Capsaicin
- SNI:
-
Spared nerve injury
- CFA:
-
Complete Freund’s adjuvant
- PWT:
-
Paw withdrawal threshold
- PWL:
-
Paw withdrawal latency
- CPZ:
-
Capsazepine
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Funding
This work was supported by the National Natural Science Foundation of China (8147377, 81603690, 81603692), the Zhejiang Provincial Natural Science Foundation [China] (LY19H270003) and the Talent Project of the Zhejiang Association for Science and Technology (2017YCGC004).
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Ju. F., J. D. and Ji. F. conceived the presented study. Ju. F., Ji. F., J. D., X. X., X. H. and Y. J. designed and carried out the experiments. Y. L. and X. S. verified the analytical methods. B. L. and J. F. supervised the project.
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In the current study, all animal manipulation complied with institutional and Chinese governmental regulations regarding the ethical use of animals (approval no. IACUC-20180319–12).
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Communicated by Sreedharan Sajikumar.
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Fang, J., Du, J., Xiang, X. et al. SNI and CFA induce similar changes in TRPV1 and P2X3 expressions in the acute phase but not in the chronic phase of pain. Exp Brain Res 239, 983–995 (2021). https://doi.org/10.1007/s00221-020-05988-4
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DOI: https://doi.org/10.1007/s00221-020-05988-4