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NADPH-diaphorase histochemical changes in the hippocampus, cerebellum and striatum are correlated with different modalities of exercise and watermaze performances

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Abstract

Nitric oxide is involved in memory and motor learning. We investigated possible influences of exercise on spatial memory and NADPH-diaphorase (NADPH-d) histochemical activity in the hippocampus, striatum and cerebellum. Fifteen albino Swiss mice between the 22nd and 55th post-natal days were exercised in the following modalities: voluntary (V), acrobatic (A), acrobatic/voluntary (AV) and forced (F) and compared to inactive group (I). After the exercise period, all subjects were tested in the water maze for 3 days. Animal brains were processed for NADPH-d histochemistry. Densitometry of the neuropil of the hippocampus, striatum and cerebellum and morphometric analysis of NADPHd+ type I neurons of the striatum were done. Exercise groups presented higher levels of NADPH-d activity in the molecular and polymorphic layers of dentate gyrus and lacunosum molecular layer of CA1. The A group presented higher NADPH-d activity in the cerebellar granular layer than all other groups. Branching points and dendritic segment densities of NADPH-d type I neurons were higher in V, A and AV than in F and I groups. Exercise groups revealed best performances on water maze tests. Thus, different modalities of exercise increases in different proportions for the nitrergic activity in the hippocampus, striatum and cerebellum, and these changes seem to be beneficial to spatial memory

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Acknowledgments

We are in debt with Dr. Aglai Pena Barbosa de Sousa for careful analysis of the manuscript and suggestions, and with Dr. Manuel Ayres for statistical assistance. This work has been funded by CNPq, FINEP and PROPESP-UFPA.

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Authors and Affiliations

  1. Department of Morphology, Federal University of Pará, Augusto Correa 01, 66075-010, Belém, Brazil

    João Bento Torres, Jarila Assunção, José Augusto Farias, Rafael Kahwage, Nara Lins, Aline Passos, Amanda Quintairos, Nonata Trévia & Cristovam Wanderley Picanço Diniz

  2. Department of Morphology, Federal University of Pará, Conjunto Médice I, Maracanã 564, 66620-260, Belém Pará, Brazil

    João Bento Torres

  3. Department of Morphology, Federal University of Pará, Rua 20 de Fevereiro 889, 66075-335, Belém Pará, Brazil

    Jarila Assunção

  4. Department of Morphology, Federal University of Pará, Av Mosqueiro 175, Conj Médice II, 66620-880, Belém Pará, Brazil

    José Augusto Farias

  5. Department of Morphology, Federal University of Pará, Av Pe Eutíquio 1080 Ap 2201, 66050-350, Belém Pará, Brazil

    Rafael Kahwage

  6. Department of Morphology, Federal University of Pará, Tv Perebebuí, Conjunto Santos Dumont II, 51, 66087-830, Belém Pará, Brazil

    Nara Lins

  7. Department of Morphology, Federal University of Pará, Conj Medice II, Rua Chaves 125, 66620-060, Belém Pará, Brazil

    Aline Passos

  8. Department of Morphology, Federal University of Pará, Av Conselheiro Furtado 1788 Ap 503, 66040-100, Belém Pará, Brazil

    Amanda Quintairos

  9. Department of Morphology, Federal University of Pará, José Bonifácio 788 apt. 602, 66063-010, Belém Pará, Brazil

    Nonata Trévia

  10. Department of Morphology, Federal University of Pará, Pass. São Pedro 99, Ananindeua, Pará, Brazil

    Cristovam Wanderley Picanço Diniz

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  1. João Bento Torres
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  2. Jarila Assunção
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  9. Cristovam Wanderley Picanço Diniz
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Correspondence to Cristovam Wanderley Picanço Diniz.

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Torres, J.B., Assunção, J., Farias, J.A. et al. NADPH-diaphorase histochemical changes in the hippocampus, cerebellum and striatum are correlated with different modalities of exercise and watermaze performances. Exp Brain Res 175, 292–304 (2006). https://doi.org/10.1007/s00221-006-0549-9

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