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Investigating the in vitro hypoglycaemic and antioxidant properties of Citrus × clementina Hort. juice

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Abstract

Citrus × clementina juice obtained from fruits collected in three different areas (flood plain, hill and coastal plain) was investigated for the chemical composition, radical scavenging properties (DPPH and ABTS tests), and α-amylase and α-glucosidase inhibitory activity. Neohesperidin (72.96–116.50 mg/100 mL), hesperidin (55.24–69.52 mg/100 mL) and narirutin (7.21–12.13 mg/100 mL) are the main flavonoids identified by HPLC analyses. In carbohydrate hydrolysing enzymes inhibitory activity tests, samples showed higher potency against α-glucosidase. Juice from hill was the most active with an IC50 value of 77.79 μg/mL. Data on the radical scavenging activity revealed the following trend of potency flood plain > coastal plain > hill. These results could help farmers to select fruits for different industrial purpose such as functional food and matrix to extract nutraceutical products.

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Abbreviations

ABTS:

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)

ADA:

American Diabetes Association

DPPH:

2,2-diphenyl-1-picrylhydrazyl

JNK:

C-Jun NH2-terminal kinase

PCA:

Principal component analysis

ROS:

Reactive oxygen species

T1DM:

Type 1 diabetes

T2DM:

Type 2 diabetes

TNF-α:

Tumour-necrosis factor-α

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Acknowledgements

The authors are grateful to Falco farms for supplying the samples.

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Authors and Affiliations

  1. Department of Pharmacy, Health Science and Nutrition, University of Calabria, Via P. Bucci, Edificio Polifunzionale, 87036, Rende, Italy

    Monica R. Loizzo, Mariarosaria Leporini, Vincenzo Sicari, Tiziana Falco & Rosa Tundis

  2. Department of Agraria, University “Mediterranea” of Reggio Calabria, Località Feo di Vito, 89122, Reggio Calabria, Italy

    Teresa M. Pellicanò

Authors
  1. Monica R. Loizzo
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  2. Mariarosaria Leporini
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  3. Vincenzo Sicari
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  4. Tiziana Falco
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  5. Teresa M. Pellicanò
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  6. Rosa Tundis
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Correspondence to Monica R. Loizzo.

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The study was not specifically funded.

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The authors declare that there are no conflicts of interest.

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Loizzo, M.R., Leporini, M., Sicari, V. et al. Investigating the in vitro hypoglycaemic and antioxidant properties of Citrus × clementina Hort. juice. Eur Food Res Technol 244, 523–534 (2018). https://doi.org/10.1007/s00217-017-2978-z

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  • DOI: https://doi.org/10.1007/s00217-017-2978-z

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