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Codonopsis lanceolata and Nelumbo nucifera Gaertn. root extracts for functional food: metabolic profiling by MS, FTIR and fluorescence and evaluation of cytotoxicity and anti-obesity properties on 3T3-L1 cell line

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Abstract

The effects of bioactivity, binding properties, anti-obesity and antioxidant enzyme activities in different fractions from the roots of Codonopsis lanceolata [C. lanceolata (CL)] in comparison with Nelumbo nucifera Gaertn. [N. nucifera Gaertn (NN)] were evaluated in vitro. The rate of cell survival progressively decreased in a dose-dependent manner. Butyl alcohol fraction of CL exhibited a pronounced cytotoxic effect (75.73 %) comparable to that of hexane (79.82 %), following by methylene chloride (84.02 %), ethyl acetate (87.62 %) and water fractions (86.30 %) at the same concentration at 200 μg/mL on 3T3-L1 cells. Confluent 3T3-L1 cells were treated with 50 µg/mL of CL and NN extracts and then stained with Oil Red O solution. Inhibitory degree of lipid accumulation against these extracts showed a significant level compared with the control sample. Both lipid accumulation and adipocyte differentiation showed relatively high effects in methyl chloride fraction. Comparative results of cytotoxicity and inhibitory degree of lipid accumulation were obtained with extracts of N. nucifera Gaertn. roots. The root extract of C. lanceolata had the significantly highest SOD enzyme activity of 84.5 % in ethyl acetate fraction and the lowest in water (57.9 %). Similar correlations were found for CAT, APX and POD activities as the highest in ethyl acetate partition layer compared with the other fractions. The binding properties were determined by interaction of polyphenols extracts of CL and NN with human serum albumin. Overall results of properties of these two plants suggest that the roots of C. lanceolata and N. nucifera Gaertn. may assist in the potential biological activity on anti-obesity, cytotoxic, antioxidant and binding capacities as a part of functional food.

Graphical Abstract

CL, NN, Codonopsis lanceolata, Nelumbo nucifera Gaertn. I, II, Effects of solvent fractions on lipid accumulation in 3T3-L1 cells of CL, NN. Con, control; MC, methylene chloride fraction; III, IV, 3D-fluorescence spectra and corresponding contour maps (III, IV) of polyphenols of CL and NN in interaction with human serum albumin.

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References

  1. Joh EH, Lee IA, Han SJ, Chae SJ, Kim DH (2010) Lancemaside A ameliorates colitis by inhibiting NF-jB activation in TNBS-induced colitis mice. Int J Colorectal Dis 25:545–551

    Article  Google Scholar 

  2. He JY, Ma N, Zhu S, Komatsu K, Li ZY, Fu WM (2015) The genus Codonopsis (Campanulaceae): a review of phytochemistry, bioactivity and quality control. J Nat Med 69:1–21

    Article  Google Scholar 

  3. Hossen MJ, Kim MY, Kim JH, Cho JY (2016) Codonopsis lanceolata: a review of its therapeutic potentials. Phytother Res 30:347–356

    Article  CAS  Google Scholar 

  4. Wang L, Xu LH, Rasmussen SK, Wang MH (2011) Codonopsis lanceolata extract induces G0/G1 arrest and apoptosis in human colon tumor HT-29 cells—involvement of ROS generation and polyamine depletion. Food Chem Toxicol 49:149–154

    Article  CAS  Google Scholar 

  5. Lee Y-J, Kim D-B, Lee JS, Cho J-H, Kim B-K, Choi H-S, Lee B-Y, Lee O-H (2013) Antioxidant activity and anti-adipogenic effects of wild herbs mainly cultivated in Korea. Molecules 18:12937–12950

    Article  CAS  Google Scholar 

  6. Park Y-S, Towantakavanit K, Namiesnik J, Sliwka-Kaszynska M, Kusznierewicz B, Kozlowska-Tylingo K, Leontowicz H, Leontowicz M, Matrinez-Ayala AL, Gorinstein S (2009) Partial characterization of three Korean white lotus cultivars. J Agric Food Chem 57:4391–4397

    Article  CAS  Google Scholar 

  7. Park Y-S, Towantakavanit K, Kowalska T, Jung S-T, Ham K-S, Heo B-G, Cho J-Y, Yun J-G, Kim H-J, Gorinstein S (2009) Bioactive compounds and antioxidant and antiproliferative activities of Korean white lotus cultivars. J Med Food 12:1057–1064

    Article  CAS  Google Scholar 

  8. Rahul BB, Kamlesh KB (2007) Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov Today 12:879–889

    Article  Google Scholar 

  9. Bhardwaj A, Modi KP (2016) A review on therapeutic potential of Nelumbo nucifera (Gaertn): the sacred lotus. Int J Pharm Sci Res 7:42–54

    Article  CAS  Google Scholar 

  10. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann Rev Plant Biol 5:373–399

    Article  Google Scholar 

  11. Zhou BY, Guo ZF, Liu ZL (2005) Effects of abscisic acid on antioxidant systems of Stylosanthes guianensis (Aublet) Sw. under chilling stress. Crop Sci 45:599–605

    Article  CAS  Google Scholar 

  12. Kang HM, Saltveit ME (2002) Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid. Physiol Plant 115:571–576

    Article  CAS  Google Scholar 

  13. Asada K (1994) Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH, Mullineaux PM (eds) Causes of photooxidative stress and amelioration of defense systems in plants. CRC Press, London, pp 77–105

    Google Scholar 

  14. Wojtaszek P (1997) Oxidative burst: an early plant response to pathogen infection. Biochem J 322:681–692

    Article  CAS  Google Scholar 

  15. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

    Article  CAS  Google Scholar 

  16. Park Y-S, Polovka M, Suhaj M, Ham K-S, Kang S-G, Park Y-K, Arancibia-Avila P, Toledo F, Robles Sanchez M, Gorinstein S (2015) The postharvest performance of kiwi fruit after long cold storage. Eur Food Res Technol 241:601–613

    Article  CAS  Google Scholar 

  17. Bae JH, Park YJ, Namiesnik J, Gülçin I, Kim T-C, Kim HC, Heo BG, Gorinstein S, Ku Y-G (2016) Effects of artificial lighting on bioactivity of sweet red pepper (Capsicum annuum L.). Int J Food Sci Technol 51:1378–1385

    Article  CAS  Google Scholar 

  18. Gomez-Romero M, Zurek G, Schneider B, Baessmann C, Segura-Carretero A, Fernandez-Gutiérrez A (2011) Automated identification of phenolics in plant derived foods by using library search approach. Food Chem 124:379–386

    Article  CAS  Google Scholar 

  19. Park YS, Namieśnik J, Vearasilp K, Leontowicz H, Leontowicz M, Barasch D, Nemirovski A, Trakhtenberg S, Gorinstein S (2014) Bioactive compounds and the antioxidant capacity in new kiwi fruit cultivars. Food Chem 165:354–361

    Article  CAS  Google Scholar 

  20. Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods Enzymol 299:152–178

    Article  CAS  Google Scholar 

  21. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237

    Article  CAS  Google Scholar 

  22. Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70–76

    Article  CAS  Google Scholar 

  23. Apak R, Guclu K, Ozyurek M, Karademir SE (2004) Novel total antioxidant capacity index for dietary polyphenol and vitamin C and E, using their cupric ion reducing capacity in the presence of neocuproine: CUPRAC method. J Agric Food Chem 52:7970–7981

    Article  CAS  Google Scholar 

  24. Mishra NP, Mishra RK, Singhal GS (1993) Changes in the activities of anti-oxidant enzymes during exposure of intact wheat leaves to strong visible light at different temperatures in the presence of protein synthesis inhibitors. Plant Physiol 102:903–910

    Article  CAS  Google Scholar 

  25. Chen GX, Asada K (1989) Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol 30:987–998

    Article  CAS  Google Scholar 

  26. Egley GH, Paul RN, Vaughn KC, Duke SO (1983) Role of peroxidase in the development of water-impermeable seed coats in Sidaspinos L. Planta 157:224–232

    Article  CAS  Google Scholar 

  27. Choi H-K, Won E-K, Jang YP, Choung S-Y (2013) Antiobesity effect of Codonopsis lanceolata in high-calorie/high-fat-diet-induced obese rats. Evid Based Complement Alternat Med eCAM. doi:10.1155/2013/210297

    Google Scholar 

  28. Heo B-G, Park Y-J, Park Y-S, Bae J-H, Cho J-Y, Park K, Jastrzebski Z, Gorinstein S (2014) Anticancer and antioxidant effects of extracts from different parts of indigo plant. Ind Crops Prod 56:9–16

    Article  CAS  Google Scholar 

  29. Kim HG, Bae JH, Jastrzebski Z, Cherkas A, Heo BG, Gorinstein S, Ku YG (2016) Binding, antioxidant and anti-proliferative properties of bioactive compounds of sweet paprika (Capsicum annuum L.). Plant Foods Hum Nutr 71:129–136

    Article  CAS  Google Scholar 

  30. Yin Y, Shim TH, Sa JH, Lohakare J, Wang MH (2010) Comparison of chemical compositions and biological activities between two varieties of Codonopsis lanceolata. Hortic Environ Biotechnol 51:123–129

    CAS  Google Scholar 

  31. You JS, Lee YJ, Kim KS, Kim SH, Chang KJ (2014) Anti-obesity and hypolipidemic effects of Nelumbo nucifera seed ethanol extract in human pre-adipocytes and rats fed a high-fat diet. J Sci Food Agric 94:568–575

    Article  CAS  Google Scholar 

  32. Tsuruta Y, Nagao K, Kai S, Tsuge K, Yoshimura T, Koganemaru K, Yanagita T (2011) Polyphenolic extract of lotus root alleviates hepatic steatosis in obese diabetic db/db mice. Lipids Health Dis 10:202–206

    Article  CAS  Google Scholar 

  33. Kim NY, Chae HS, Lee IS, Kim DS, Seo KT, Park SJ (2010) Analysis of chemical composition and antioxidant activity of Codonopsis lanceolata skin. Han’guk Sikp’um Yongyang Kwahak Hoechi 39:1627–1633

    CAS  Google Scholar 

  34. Ekinci FY, Baser GM, Özcan E, Üstünda ÖG, Korachi M, Sofu A, Blumberg JB, Chen C-y O (2016) Characterization of chemical, biological, and antiproliferative properties of fermented black carrot juice, shalgam. Eur Food Res Technol 242:1355–1368

    Article  CAS  Google Scholar 

  35. Sha L (2014) Anti-obesity effects of Acorus gramineus Soland. Rhizome in 3T3-L1 preadipocytes and C57BL/6N mice. Thesis for the degree of Master of Science. Andong National University (Korea)

  36. Cha A, Choi Y, Jin Y, Sung MK, Koo YC, Lee KW, Park T (2012) Antilipogenic and anti-inflammatory activities of Codonopsis lanceolata in mice hepatic tissues after chronic ethanol feeding. J Biomed Biotechnol. doi:10.1155/2012/141395

    Google Scholar 

  37. Velusami CC, Agarwal A, Mookambeswaran V (2013) Effect of Nelumbo nucifera petal extracts on lipase, adipogenesis, adipolysis, and central receptors of obesity. Evid Based Complement Alternat Med. doi:10.1155/2013/145925

    Google Scholar 

  38. Najami N, Tibor J, Barriah W, Kayam G, Moshe T, Guy M, Volokita M (2008) Ascorbate peroxidase gene family in tomato: its identification and characterization. Mol Genet Genom 279:171–182

    Article  CAS  Google Scholar 

  39. Gaspar T, Penel C, Castillo FJ, Greppin H (1985) A two step control of basic and acid peroxidases and its significance for growth and development. Plant Physiol 64:418–423

    Article  CAS  Google Scholar 

  40. Asish KP, Anath BD (2005) Salt tolerance and salinity effects on plants. A review. Ecotoxicol Environ Saf 60:324–349

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by High Value-added Food Technology Development Program of IPET, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. The authors are thankful to Dr. Elena Katrich (School of Pharmacy, Hebrew University of Jerusalem) for her technical assistance in determination of antioxidant activity and fluorescence.

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Authors and Affiliations

  1. WELLPHYTO Co. Ltd., BI Center, GIST, Gwangju, 61005, Korea

    Hee-Ock Boo

  2. Faculty of Food Nutrition and Cookery, Woosong Information College, Daejeon, 34606, Korea

    Hag-Hyun Kim

  3. Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah Medical School, 91120, Jerusalem, Israel

    Dinorah Barasch, Alina Nemirovski & Shela Gorinstein

  4. Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, 28644, Korea

    Moon-Soon Lee

  5. Department of Horticulture Industry, College of Life Science and Resource, Wonkwang University, Iksan, 54538, Korea

    Yang-Gyu Ku

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  1. Hee-Ock Boo
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Correspondence to Shela Gorinstein or Yang-Gyu Ku.

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Boo, HO., Kim, HH., Barasch, D. et al. Codonopsis lanceolata and Nelumbo nucifera Gaertn. root extracts for functional food: metabolic profiling by MS, FTIR and fluorescence and evaluation of cytotoxicity and anti-obesity properties on 3T3-L1 cell line. Eur Food Res Technol 243, 689–700 (2017). https://doi.org/10.1007/s00217-016-2782-1

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  • DOI: https://doi.org/10.1007/s00217-016-2782-1

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