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Hypolipidemic mechanism of gypenosides via inhibition of pancreatic lipase and reduction in cholesterol micellar solubility

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Abstract

Effect of gypenosides on porcine pancreatic lipase (PL) activity was determined in vitro. Gypenosides inhibited the PL activity in a dose-dependent manner at the concentrations of 0–1.0 mg/ml. The Lineweaver–Burk plots for gypenosides indicated that the inhibition on PL by gypenosides was noncompetitive. The conformation of PL was investigated by means of circular dichroism and fluorescence. The result revealed the decrease in α-helix contents, increase in β-sheet and the decrease in fluorescence intensity after gypenosides treatment. The incorporation of cholesterol into micelles was inhibited by gypenosides. A complex was observed by transmission electron microscopy, indicating interaction between cholesterol and gypenosides which led to the decrease in cholesterol micelle solubility. The result revealed the fact that gypenosides played a role in inhibiting PL activity and reducing intestinal cholesterol absorption via inhibition of micelle formation.

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Acknowledgments

This work was supported by the 125 Project (2012BAD33B05), and Anhui Tongling Ruipu Peony Industry Development Co., Ltd.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu Province, People’s Republic of China

    Jianhui Su, Hongxin Wang, Chaoyang Ma, Chengxiang Liu & MdRamim Tanver Rahman

  2. Anhui Tongling Ruipu Peony Industry Development Co., Ltd., Tongling, 244000, Anhui, China

    Chuanzhong Gao & Rongjing Nie

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  1. Jianhui Su
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  2. Hongxin Wang
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  3. Chaoyang Ma
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  4. Chengxiang Liu
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  6. Chuanzhong Gao
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  7. Rongjing Nie
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Correspondence to Hongxin Wang.

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Su, J., Wang, H., Ma, C. et al. Hypolipidemic mechanism of gypenosides via inhibition of pancreatic lipase and reduction in cholesterol micellar solubility. Eur Food Res Technol 242, 305–312 (2016). https://doi.org/10.1007/s00217-015-2540-9

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  • DOI: https://doi.org/10.1007/s00217-015-2540-9

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