Abstract
Dry-cured ham is a traditional meat product highly appreciated by consumers. Production of dry-cured ham is a time-consuming process which varies between different ham types. There are many factors affecting the final characteristics of dry-cured ham. The quality of the raw material and the process conditions mainly influence the rate and the extent of biochemical reactions which are in turn responsible for the formation of specific flavor and texture. This review paper highlights the characteristics of the raw material, the enzymatic and chemical processes taking place during dry-cured ham manufacture and the compounds formed by these reactions. The rates of the enzymatic changes from fresh meat to the stage of final product are also described.
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Benedini R, Parolari G, Toscani T, Virgili R (2012) Sensory and texture properties of Italian typical dry-cured hams as related to maturation time and salt content. Meat Sci 90(2):431–437
Buscailhon S, Monin G (1994) Déterminisme des qualités sensorielles du jambon sec. Chapitre 1: evolution de la composition et des qualités sensorielles du jambon sec au cours de la fabrication. Viandes et Produits carnés 15:23–34
Laureati M, Buratti S, Giovanelli G, Corazzin M, Lo Fiego DP, Pagliarini E (2014) Characterization and differentiation of Italian Parma, San Daniele and Toscano dry-cured hams: a multi-disciplinary approach. Meat Sci 96(1):288–294
Toldra F, Flores M (1998) The role of muscle proteases and lipases in flavor development during the processing of dry-cured ham. Crit Rev Food Sci Nutr 38:331–352
Virgili R, Saccani G, Gabba L, Tanzi E, Soresi Bordini C (2007) Changes of free amino acids and biogenic amines during extended ageing of Italian dry-cured ham. LWT Food Sci Technol 40(5):871–878
Toldra F (2002) Dry-cured meat products. Food and Nutritional Press, Trumbull
Wang ZY, Gao XG, Zhang JH, Zhang DQ, Ma CW (2013) Changes of intramuscular fat composition, lipid oxidation and lipase activity in biceps femoris and semimembranosus of Xuanwei Ham during controlled salting stages. J Integr Agric 12(11):1993–2001
Jurado A, Garcia C, Timon ML, Carrapiso AI (2007) Effect of ripening time and rearing system on amino acid-related flavour compounds of Iberian ham. Meat Sci 75(4):585–594
Hidalgo FJ, Zamora R (2004) Strecker-type degradation produced by the lipid oxidation products 4,5-epoxy-2-alkenals. J Agric Food Chem 52(23):7126–7131
Pripis-Nicolau L, de Revel G, Bertrand A, Maujean A (2000) Formation of flavor components by the reaction of amino acid and carbonyl compounds in mild conditions. J Agric Food Chem 48(9):3761–3766
Carrapiso AI, Ventanas J, Garcia C (2002) Characterization of the most odor-active compounds of Iberian ham headspace. J Agric Food Chem 50(7):1996–2000
Mujumdar AS (2006) Handbook of industrial drying, 3rd edn. CRS Press, Boca Raton
Bermudez R, Franco D, Carballo J, Sentandreu MA, Lorenzo JM (2014) Influence of muscle type on the evolution of free amino acids and sarcoplasmic and myofibrillar proteins through the manufacturing process of Celta dry-cured ham. Food Res Int 56:226–235
Flores M, Ingram DA, Bett KL, Toldra F, Spanier AM (1997) Sensory characteristics of Spanish “Serrano” dry-cured ham. J Sens Stud 12(3):169–179
Motilva M-J, Toldra F, Flores J (1992) Assay of lipase and esterase activities in fresh pork meat and dry-cured ham. Z Lebensm Unters Forch 195:446–450
Ruiz-Ramirez J, Arnau J, Serra X, Gou P (2006) Effect of pH24, NaCl content and proteolysis index on the relationship between water content and texture parameters in biceps femoris and semimembranosus muscles in dry-cured ham. Meat Sci 72(2):185–194
Toldra F (2006) The role of muscle enzymes in dry-cured meat products with different drying conditions. Trend Food Sci Technol 17(4):164–168
Toldra F, Cervero MC, Part C (1993) Porcine aminopeptidase activity as affected by curing agents. J Food Sci 58(4):724–726
Toldra F, Rico E, Flores J (1992) Activities of pork muscle proteases in model cured meat systems. Biochimie 74(3):291–296
Toldra F (1998) Proteolysis and lipolysis in flavour development of dry-cured meat products. Meat Sci 49:S101–S110
Toldra F, Flores M, Sanz Y (1997) Dry-cured ham flavour: enzymatic generation and process influence. Food Chem 59(4):523–530
Zhao GM, Zhou GH, Tian W, Xu XL, Wang YL, Luo X (2005) Changes of alanyl aminopeptidase activity and free amino acid contents in biceps femoris during processing of Jinhua ham. Meat Sci 71(4):612–619
Clemente G, Bon J, Sanjuan N, Mulet A (2011) Drying modelling of defrosted pork meat under forced convection conditions. Meat Sci 88(3):374–378
Gou P, Comaposada J, Arnau J (2004) Moisture diffusivity in the lean tissue of dry-cured ham at different process times. Meat Sci 67(2):203–209
Protected Destination of Origin. Prosciutto di Parma (Parma Ham) (1992) Specifications and Dossier pursuant to Article 4 of Council Regulation EEC No. 2081/92
Prosciutto Toscano D.O.P. (1996) Disciplinare di produzione della denominazione di origine protetta prosciutto Toscano. Regolamento CE No. 1263/96
Carrapiso AI, García C (2008) Effect of the Iberian pig line on dry-cured ham characteristics. Meat Sci 80(2):529–534
Flores M, Aristoy MC, Antequera T, Barat JM, Toldra F (2009) Effect of prefreezing hams on endogenous enzyme activity during the processing of Iberian dry-cured hams. Meat Sci 82(2):241–246
Haseth TT (2012) Salting av spekeskinke, Animalia. http://www.animalia.no/Slakt–kjott–og-eggkvalitet/Spekematproduksjon/Aktuelt-og-fagstoff/Salting-av-spekeskinke. Accessed 10 Apr 2015
Bayonne dry-cured ham: less salt for improved technological and slicing yields (2010) The French National Institute For Agricultural Research (INRA). http://www.inra.fr/en/Partners-and-Agribusiness/Results-Innovations-Transfer/All-the-news/Bayonne-dry-cured-ham2/. Accessed 10 Apr 2015
Monin G, Marinova P, Talmant A, Martin JF, Cornet M, Lanore D, Grasso F (1997) Chemical and structural changes in dry-cured hams (Bayonne hams) during processing and effects of the dehairing technique. Meat Sci 47(1–2):29–47
Theron L, Chevarin L, Robert N, Dutertre C, Sante-Lhoutellier V (2009) Time course of peptide fingerprints in semimembranosus and biceps femoris muscles during Bayonne ham processing. Meat Sci 82(2):272–277
Zhao GM, Tian W, Liu YX, Zhou GH, Xu XL, Li MY (2008) Proteolysis in biceps femoris during Jinhua ham processing. Meat Sci 79(1):39–45
Zhou GH, Zhao GM (2007) Biochemical changes during processing of traditional Jinhua ham. Meat Sci 77(1):114–120
Toldra F (2006) Dry-cured ham. In: Hui YH (ed) Handbook of food science, technology, and engineering, 4th edn. CRC Press, Boca Raton
Toldra F, Hui YH, Astiasaran I, Sebranek J, Talon R (2014) Handbook of fermented meat and poultry, 2nd edn. Wiley, Blackwell
Arnau J, Guerrero L, Gou P (2003) Effect of meat pH and the amount of added nitrite and nitrate on color uniformity of dry-cured hams. Fleischwirtsch Int 18:31–32
Honikel K-O (2008) The use and control of nitrate and nitrite for the processing of meat products. Meat Sci 78(1–2):68–76
Toldra F, Aristoy MA, Flores M (2009) Relevance of nitrate and nitrite in dry-cured ham and their effects on aroma development. Grasa Aceite 60(3):291–296
Cammack R, Joannou CL, Cui XY, Torres Martinez C, Maraj SR, Hughes MN (1999) Nitrite and nitrosyl compounds in food preservation. Biochim Biophys Acta 1411:475–488
Morales P, Martinez A, Haza AI (2000) N-nitrosaminas en los alimentos de riesgo para la salud (I). Alimentación, equipos y tecnologia 7:153–160
Demeyer D, Raemaekers M, Rizzo A, Holck A, De Smedt A, Ten Brink B, Hagen B, Montel C, Zanardi E, Murbrekk E, Leroy F, Vandendriessche F, Lorentsen K, Venema K, Sunesen L, Stahnke L, De Vuyst L, Talon R, Chizzolini R, Eerola S (2000) Control of bioflavour and safety in fermented sausages: first results of a European project. Food Res Int 33(3–4):171–180
Pearson AM, Gillett TA (1996) Processed meats. Springer, New York
Damodaran S, Parkin KL, Fennema OR (2008) Fennema’s food chemistry. CRC Press, Boca Raton
Virgili R, Schivazappa C, Parolari G, Bordini CS, Degni M (1998) Proteases in fresh pork muscle and their influence on bitter taste formation in dry-cured ham. J Food Biochem 22(1):53–63
Parreno M, Cusso R, Gil M, Sarraga C (1994) Development of cathepsin B, L and H activities and cystatin-like activity during two different manufacturing processes for Spanish dry-cured ham. Food Chem 49(1):15–21
Buscailhon S, Gandemer G, Monin G (1994) Time-related changes in intramuscular lipids of French dry-cured ham. Meat Sci 37(2):245–255
Flores J, Bermell S, Nieto P (1985) Evaluación de la calidad de los productos cárnicos. III. Jamón curado. Revista de Agroquímica y Tecnología de los Alimentos 25:400–407
Harkouss R, Astruc T, Lebert A, Gatellier P, Loison O, Safa H, Portanguen S, Parafita E, Mirade P-S (2015) Quantitative study of the relationships among proteolysis, lipid oxidation, structure and texture throughout the dry-cured ham process. Food Chem 166:522–530
The University of Nebraska (2015) Porcine myology, Pork.org. http://porcine.unl.edu/porcine2005/pages/index.jsp/. Accessed 10 Apr 2015
Mglinec A (2010) Food technology. Troickiy most, Saint-Petersburg
Vinnikova L (2006) Technology of meat and meat products. INKOS, Kiev
Larrea V, Perez-Munuera I, Hernando I, Quiles A, Llorca E, Lluch MA (2007) Microstructural changes in Teruel dry-cured ham during processing. Meat Sci 76(3):574–582
Etherington DJ, Taylor MA, Wakefield DK, Cousins A, Dransfield E (1990) Proteinase (Cathepsin B, D, L and Calpains) levels and conditioning rates in normal, electrically stimulated and high-ultimate-pH chicken muscle. Meat Sci 28(2):99–109
Goll DE, Otsuka Y, Nagainis PA, Shannon JD, Sathe SK, Muguruma M (1983) Role of muscle proteinases in maintenance of muscle integrity and mass. Food Biochem 7(3):137–177
Koohmaraie M, Babiker AS, Merkel RA, Dutson TR (1988) Role of Ca++-dependent proteases and lysosomal enyzmes in postmortem changes in bovine skeletal muscle. J Food Sci 53(5):1253–1257
Koohmaraie M (1992) The role of Ca2+-dependent proteases (calpains) in post mortem proteolysis and meat tenderness. Biochimie 74(3):239–245
Ouali A, Garrel N, Obled A, Deval C, Valin C, Penny IF (1987) Comparative action of cathepsins D, B, H, L and of a new lysosomal cysteine proteinase on rabbit myofibrils. Meat Sci 19(2):83–100
Ouali A (1992) Proteolytic and physicochemical mechanisms involved in meat texture development. Biochimie 74(3):251–265
Zeece MG, Katoh K (1989) Cathepsin D and its effect on myofibrillar proteins: a review 1. J Food Biochem 13(3):157–178
Barrett AJ (1987) The cystatins: a new class of peptidase inhibitors. Trend Biochem Sci 12:193–196
Turk V, Bode W (1991) The cystatins: protein inhibitors of cysteine proteinases. FEBS Lett 285(2):213–219
Parreno M, Sarraga C, Gil M, Cusso R (1990) Actividad calpaina en el proceso de maduracion del jamon curado. In Proceedings of the Ninth Congress (BIOTEC 90), Murcia, Spain
Sarraga C, Gil M, Garcia-Regueiro JA (1993) Comparison of calpain and cathepsin (B, L and D) activities during dry-cured ham processing from heavy and light large white pigs. J Sci Food Agric 62(1):71–75
Jolivel V, Arthaud S, Botia B, Portal C, Delest B, Clave G, Leprince J, Romieu A, Renard PY, Touzani O, Ligeret H, Noack P, Massonneau M, Fournier A, Vaudry H, Vaudry D (2014) Biochemical characterization of a Caspase-3 far-red fluorescent probe for non-invasive optical imaging of neuronal apoptosis. J Mol Neurosci 54(3):451–462
Kemp CM, Bardsley RG, Parr T (2006) Changes in caspase activity during the postmortem conditioning period and its relationship to shear force in porcine longissimus muscle. J Anim Sci 84(10):2841–2846
Toldra F, Nollet LML (2012) Proteomics in foods: principles and applications. Springer, New York
Dura MA, Flores M, Toldra F (2004) Effect of Debaryomyces spp. on the proteolysis of dry-fermented sausages. Meat Sci 68(2):319–328
Scannell AGM, Kenneally PM, Arendt EK (2004) Contribution of starter cultures to the proteolytic process of a fermented non-dried whole muscle ham product. Int J Food Microbiol 93(2):219–230
Armero E, Barbosa JA, Toldra F, Baselga M, Pla M (1999) Effects of the terminal sire type and sex on pork muscle cathepsins (B, B + L and H), cysteine proteinase inhibitors and lipolytic enzyme activities. Meat Sci 51(2):185–189
Armero E, Flores M, Toldra F, Barbosa J-A, Olivet J, Pla M, Baselga M (1999) Effects of pig sire type and sex on carcass traits, meat quality and sensory quality of dry-cured ham. J Sci Food Agric 79(9):1147–1154
Flores M, Romero J, Aristoy MC, Flores J, Toldra F (1994) Differences in muscle proteolytic activities among pork breed types. Sci Des Aliment 14:469–474
Rosell CM, Toldra F (1998) Comparison of muscle proteolytic and lipolytic enzyme levels in raw hams from Iberian and White pigs. J Sci Food Agric 76(1):117–122
Toldra F, Flores M, Aristoy MC, Virgili R, Parolari G (1996) Pattern of muscle proteolytic and lipolytic enzymes from light and heavy pigs. J Sci Food Agric 71(1):124–128
Toldra F, Reig M, Hernandez P, Navarro J-L (1996) Lipids from pork meat as related to a healthy diet. Recent Res Dev Nutr 1:79–86
Zhao GM, Zhou GH, Wang YL, Xu XL, Huan YJ, Wu JQ (2005) Time-related changes in cathepsin B and L activities during processing of Jinhua ham as a function of pH, salt and temperature. Meat Sci 70(2):381–388
Morales R, Serra X, Guerrero L, Gou P (2007) Softness in dry-cured porcine biceps femoris muscles in relation to meat quality characteristics and processing conditions. Meat Sci 77(4):662–669
Cordero MR, Zumalacarregui JM (2000) Characterization of micrococcaceae isolated from salt used for Spanish dry-cured ham. Lett Appl Microbiol 31(4):303–306
Luccia AD, Picariello G, Cacace G, Scaloni A, Faccia M, Liuzzi V, Alviti G, Musso SS (2005) Proteomic analysis of water soluble and myofibrillar protein changes occurring in dry-cured hams. Meat Sci 69(3):479–491
Skrlep M, Mandelc S, Javornik B, Sante-Lhoutellier V, Pere G, Marjeta C-P (2010) Green ham pH value affects proteomic profile of dry-cured ham. Ital J Anim Sci 9(29):153–156
Arnau J, Guerrero L, Sarraga C (1998) The effect of green ham pH and NaCl concentration on cathepsin activities and the sensory characteristics of dry-cured hams. J Sci Food Agric 77(3):387–392
Schivazappa C, Degni M, Nanni Costa L, Russo V, Buttazzoni L, Virgili R (2002) Analysis of raw meat to predict proteolysis in Parma ham. Meat Sci 60(1):77–83
Toldra F, Etherington DJ (1988) Examination of cathepsins B, D, H and L activities in dry-cured hams. Meat Sci 23(1):1–7
Parma Ham. Well-being and diet. Nutritional values (2012) Consorzio del Prosciutto di Parma. http://www.prosciuttodiparma.com/pdf/Parma%20Ham%20Well-being%20and%20diet.pdf. Accessed 10 Apr 2015
Cordoba JJ, Antequera T, Garcia C, Ventanas J, Lopez Bote C, Asensio MA (1994) Evolution of free amino acids and amines during ripening of Iberian cured ham. J Agric Food Chem 42(10):2296–2301
Martin L, Antequera T, Ventanas J, Benı́tez-Donoso R, Cordoba JJ (2001) Free amino acids and other non-volatile compounds formed during processing of Iberian ham. Meat Sci 59(4):363–368
Ruiz J, Garcıia C, Carmen Dıiaz MAD, Cava R, Florencio Tejeda J, Ventanas J (1999) Dry cured Iberian ham non-volatile components as affected by the length of the curing process. Food Res Int 32(9):643–651
Belfrage P, Frederikson G, Stralfors P, Thornqvist H (1984) Adipose tissue lipases. Elsevier Science Publisher, Amsterdam
Andres AI, Cava R, Martin D, Ventanas J, Ruiz J (2005) Lipolysis in dry-cured ham: influence of salt content and processing conditions. Food Chem 90(4):523–533
Fiego DPL, Macchioni P, Santoro P, Pastorelli G, Corino C (2005) Effect of dietary conjugated linoleic acid (CLA) supplementation on CLA isomers content and fatty acid composition of dry-cured Parma ham. Meat Sci 70:285–291
Motilva M-J, Toldra F, Nieto P, Flores J (1993) Muscle lipolysis phenomena in the processing of dry-cured ham. Food Chem 48(2):121–125
Timon ML, Ventanas J, Carrapiso AI, Jurado A, Garcıia C (2001) Subcutaneous and intermuscular fat characterisation of dry-cured Iberian hams. Meat Sci 58(1):85–91
Huang Y, Li H, Huang T, Li F, Sun J (2014) Lipolysis and lipid oxidation during processing of Chinese traditional smoke-cured bacon. Food Chem 149:31–39
Martıin L, Cordoba JJ, Ventanas J, Antequera T (1999) Changes in intramuscular lipids during ripening of Iberian dry-cured ham. Meat Sci 51(2):129–134
Gandemer G (2002) Lipids in muscles and adipose tissues, changes during processing and sensory properties of meat products. Meat Sci 62(3):309–321
Vestergaard CS, Schivazappa C, Virgili R (2000) Lipolysis in dry-cured ham maturation. Meat Sci 55(1):1–5
Acknowledgments
The work was supported by the Research Council of Norway (Project 225262/E40—DryMeat). Many thanks to the Food Technology Group of NTNU and the Sintef Energy Research Group for cooperation, help, and support.
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Petrova, I., Aasen, I.M., Rustad, T. et al. Manufacture of dry-cured ham: a review. Part 1. Biochemical changes during the technological process. Eur Food Res Technol 241, 587–599 (2015). https://doi.org/10.1007/s00217-015-2490-2
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DOI: https://doi.org/10.1007/s00217-015-2490-2